Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.9K
  1. Home
  3. Research Domains
  5. Chemical Sciences
  7. Macromolecular And Materials Chemistry
  9. Nanochemistry
  11. Mass Spectrometry-based Top-down Proteomics For Proteoform Profiling Of Protein Coronas

Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas

Seyed Amirhossein Sadeghi1, Fei Fang1, Reyhane Tabatabaeian Nimavard1

  • 1Department of Chemistry, Michigan State University, East Lansing, MI, USA.

Nature Protocols
|August 5, 2025

Related Experiment Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.6K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

4.5K
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

4.7K

View abstract on PubMed

Summary
This summary is machine-generated.

This study introduces capillary zone electrophoresis-mass spectrometry (CZE-MS) top-down proteomics to analyze nanoparticle protein coronas. This method accurately identifies specific protein proteoforms, crucial for nanomedicine and biomarker discovery.

Area of Science:

  • Nanomedicine
  • Proteomics
  • Biomolecular Characterization

Background:

  • The protein corona on nanoparticles (NPs) influences their therapeutic and diagnostic applications.
  • Traditional bottom-up proteomics struggles to identify specific protein proteoforms in the corona.
  • Accurate proteoform identification is vital for predicting nanomedicine behavior and advancing biomarker discovery.

Purpose of the Study:

  • To develop and present a capillary zone electrophoresis-mass spectrometry (CZE-MS) based top-down proteomics workflow.
  • To enable precise characterization of the protein proteoform landscape within nanoparticle protein coronas.
  • To overcome limitations of traditional proteomics in identifying diverse proteoforms.

Main Methods:

  • Detergent-assisted elution to recover intact proteoforms from NP surfaces.

Related Experiment Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.6K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

4.5K
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

4.7K
  • Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) for proteoform separation and identification.
  • Integration of high-field asymmetric waveform ion mobility spectrometry (FAIMS) with CZE-MS/MS for enhanced characterization.

    • Main Results:

    • Successfully identified hundreds of proteoforms from the protein corona of polystyrene NPs.
    • Demonstrated distinct protein corona proteoform profiles correlating with NP physicochemical properties.
    • Showcased the utility of FAIMS for more in-depth proteoform analysis.

    Conclusions:

    • CZE-MS top-down proteomics offers a robust method for detailed protein corona analysis.
    • This approach significantly improves the characterization of nanomedicine-biomolecule interactions.
    • The protocol facilitates accurate proteoform profiling, essential for nanomedicine development and diagnostics.