Novel High-Efficient Method to Generate Fragmented Nano- and Microfibers Enabling an Additive for Bio-Inks

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Biotechnology

Background:

• Biofabrication utilizes biopolymers and cells to create tissue mimics.
• Fiber reinforcement in bio-inks improves mechanical properties and cell viability.
• Current fiber fragmentation methods are limited by scalability and equipment needs.

Purpose of the Study:

• To introduce a novel, accessible method for controlled fiber fragmentation in biofabrication.
• To overcome limitations of existing fiber fragmentation techniques.
• To enable scalable and cost-effective production of fiber-reinforced bio-inks.

Main Methods:

• Utilized ultraviolet (UV) irradiation for controlled fiber fragmentation.
• Applied multi-modal Weibull analysis to estimate average fiber length.
• Validated the technique on polycaprolactone (PCL) and gelatin blends.

Main Results:

• UV irradiation provides controlled fiber shortening.
• Average fiber length can be accurately estimated using Weibull analysis.
• The method is cost-effective, biocompatible, and simple to implement.

Conclusions:

• UV irradiation offers a practical solution for fiber fragment production in biofabrication.
• This technique enhances the accessibility and scalability of fiber-based biofabrication.
• The developed method supports advancements in tissue engineering and regenerative medicine.