Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Analytical Methods01:21

Development of Analytical Methods

716
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
716
Catalysis02:50

Catalysis

27.6K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
27.6K
Response Surface Methodology01:16

Response Surface Methodology

267
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
267
Complement System01:27

Complement System

2.7K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
2.7K
Methods of Documentation II: POMR01:26

Methods of Documentation II: POMR

1.1K
The Problem-Oriented Medical Record (POMR) revolutionized medical record-keeping by introducing a systematic approach focusing on the patient's problems rather than merely listing symptoms. Dr. Lawrence Weed's introduction of this method in the 1960s marked a significant advancement in medical documentation. The POMR framework consists of four key components: the database, problem list, plan of care, and progress notes.
1.1K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

8.8K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nourishing Collaboration: Interdisciplinary Nutrition Education for Health Care Professionals.

The American journal of clinical nutrition·2026
Same author

Harms Reporting Was Frequently Incomplete or Discordant in Biomedical Randomized Trials Published in 2023: A Meta-epidemiological Study.

Journal of clinical epidemiology·2026
Same author

Democratizing Artificial Intelligence in Toxicology: Real-World Applications and Automated Computational Workflows.

Chemical research in toxicology·2026
Same author

Influence of Social and Demographic Factors on Retinoblastoma Outcomes in the United States: A Systematic Review.

Cochrane evidence synthesis and methods·2026
Same author

Reimagining human-centric drug development with new approach methodologies.

Science (New York, N.Y.)·2026
Same author

Associations of Social and Demographic Factors on the Outcomes of Ocular Melanoma and Other Adult Ocular Neoplasms in the United States: A Systematic Review.

Cochrane evidence synthesis and methods·2026
Same journal

New frontiers in anti-cancer drug testing: The need for a relevant In vitro testing model.

NAM journal·2026
Same journal

GenoITS: Implementation of an Integrated Testing Strategy workflow for genotoxicity using QSAR-based tools.

NAM journal·2026
Same journal

Advice for bad toxicologists.

NAM journal·2026
Same journal

Towards replacement of animal experimentation in scientific research and regulatory testing: launch of <i>NAM Journal</i>.

NAM journal·2026
Same journal

Including genetic susceptibility towards Parkinson's disease in NAM-based hazard and risk assessment of pesticides: a semi-systematic review.

NAM journal·2026
Same journal

SCCS opinion on biphenyl-2-ol and sodium 2-biphenylolate used in cosmetic products (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6)- SCCS/1669/24.

NAM journal·2026
See all related articles

Related Experiment Video

Updated: Sep 12, 2025

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods
05:34

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods

Published on: June 6, 2025

617

Complement-ARIE: Catalyzing the Development and Adoption of New Approach Methodologies.

Kristifor Sunderic1, Andrew M Wright2, Nicole Kleinstreuer2,3

  • 1National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD 20892, USA.

NAM Journal
|August 6, 2025
PubMed
Summary
This summary is machine-generated.

New approach methodologies (NAMs) offer advanced tools for research, complementing animal models. The NIH

Keywords:
Complement Animal Research In Experimentation, Complement-ARIENational Institutes of Health, NIHNew approach methodologies, novel alternative methods, NAMsin chemicoin silicoin vitromicrophysiological systems, MPS

More Related Videos

The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time
06:05

The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time

Published on: February 19, 2021

1.4K
A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting
14:43

A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

Published on: January 12, 2018

12.0K

Related Experiment Videos

Last Updated: Sep 12, 2025

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods
05:34

Applying Cheminformatics to Develop a Structure Searchable Database of Analytical Methods

Published on: June 6, 2025

617
The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time
06:05

The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time

Published on: February 19, 2021

1.4K
A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting
14:43

A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

Published on: January 12, 2018

12.0K

Area of Science:

  • Toxicology and pharmacology research.
  • Biomedical sciences and drug development.
  • Regulatory science and standardization.

Background:

  • New Approach Methodologies (NAMs) are increasingly vital in research, offering alternatives and complements to animal testing.
  • The National Institutes of Health (NIH) has significantly boosted investment in diverse NAMs, including in chemico, in silico, and in vitro systems.
  • Existing NAMs require further development, standardization, and validation for broader application.

Purpose of the Study:

  • To introduce the NIH Common Fund's Complement Animal Research In Experimentation (Complement-ARIE) program.
  • To outline the program's goals: advancing combinatorial NAMs for accurate human biology modeling, population-specific outcomes, and regulatory acceptance.
  • To detail strategic planning activities informing Complement-ARIE's implementation.

Main Methods:

  • NIH organized strategic planning, including an inter-agency retreat, public listening sessions, and literature analysis.
  • A challenge prize was utilized to stimulate innovation in NAM development.
  • Analysis of scientific literature to understand the current landscape of NAMs.

Main Results:

  • Strategic planning identified key scientific and operational opportunities and challenges for NAM implementation.
  • Findings will guide the development, standardization, validation, and regulatory use of NAMs.
  • The program aims to enhance the efficiency and effectiveness of research by complementing traditional animal models.

Conclusions:

  • The Complement-ARIE program is poised to catalyze innovation in NAMs.
  • Successful implementation will lead to broader acceptance and impact of NAMs in basic, translational, and clinical sciences.
  • These advancements are expected to transform scientific research methodologies.