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Updated: Sep 12, 2025

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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Fibroblast Growth Factor 19 Disrupts Cartilage Development Via the FGFR4/β-catenin Axis

Hao Chen1, Yujia Cui1, Jiazhou Li1

  • 1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

International Journal of Biological Sciences
|August 6, 2025
PubMed

Insights

Fibroblast growth factor 19 (FGF19) impairs cartilage development by inhibiting chondrocyte maturation and growth plate thickness. This occurs through the FGFR4/β-catenin pathway, affecting skeletal growth.

Area of Science:

  • Skeletal Biology
  • Endocrinology
  • Developmental Biology

Background:

  • Fibroblast growth factor 19 (FGF19) is implicated in skeletal metabolic disorders.
  • The specific role of FGF19 in cartilage development remains unclear.

Purpose of the Study:

  • To investigate the influence of FGF19 on cartilage development and chondrogenesis.
  • To elucidate the molecular mechanisms underlying FGF19's effects on growth plate chondrocytes.

Main Methods:

  • Utilized an ex vivo metatarsal organ model for neonatal cartilage.
  • Employed an adeno-associated virus (AAV)-FGF19 overexpression model in adolescent growth plates.
  • Assessed chondrocyte differentiation, maturation, and proliferation markers.

Main Results:

  • FGF19 impaired chondrocyte maturation in neonatal models and reduced growth plate thickness in adolescent models.
  • FGF19 decreased mesenchymal stem cell chondrogenic differentiation and chondrocyte maturation by downregulating Wnt/β-catenin signaling.
  • FGF19 signaling, via FGFR4 and β-klotho (KLB), activated Wnt antagonists (SFRP1, WIF1, DKK2), inhibiting chondrocyte proliferation and cartilage growth.

Conclusions:

  • FGF19 inhibits cartilage development and growth plate chondrogenesis through the FGFR4/β-catenin axis.
  • This study provides novel insights into the role of FGF19 in endochondral ossification and skeletal development.
Keywords:
Wnt/β-Cateninchondrogenesisfibroblast growth factor 19growth plate

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