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Updated: Sep 12, 2025

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Published on: December 29, 2023

Challenges with measuring tissue factor antigen and activity in human plasma.

Nigel Mackman¹, Ana T A Sachetto¹

¹Division of Hematology, Department of Medicine, UNC Blood Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC.

Blood Vessels, Thrombosis & Hemostasis

|August 6, 2025

Summary

Measuring tissue factor (TF) activity on extracellular vesicles (EVs) in blood shows promise for identifying thrombotic risk. Functional assays are more sensitive than antigen tests for detecting low TF levels in disease.

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Related Experiment Videos

Last Updated: Sep 12, 2025

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Published on: December 29, 2023

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Area of Science:

Biochemistry and Molecular Biology
Hematology
Cardiovascular Research

Background:

Tissue factor (TF) is a key initiator of blood coagulation, crucial for hemostasis and thrombosis.
Elevated levels of TF-positive extracellular vesicles (EVs) are linked to thrombotic diseases.
Accurate quantification of low TF levels in blood remains a significant challenge.

Purpose of the Study:

To evaluate the utility of functional TF activity assays for measuring TF on EVs.
To compare the sensitivity and specificity of different TF detection methods.
To explore TF activity on EVs as a potential biomarker for thrombotic risk.

Main Methods:

Utilized activity-based assays for enhanced sensitivity and specificity in TF detection.
Employed functional assays with and without inhibitory anti-TF antibodies to distinguish TF-dependent activity.
Assessed TF activity on isolated EVs using commercial assays.

Main Results:

Functional TF activity assays demonstrate higher sensitivity and specificity compared to antigen-based assays.
TF pathway inhibitor effectively reduces TF activity in isolated EVs.
Commercial assays for measuring TF activity on EVs are available.

Conclusions:

Measurement of TF activity on EVs isolated from plasma offers a promising approach for thrombotic risk assessment.
Functional TF assays are superior to antigen assays for detecting low TF levels in disease states.
TF activity on EVs may serve as a valuable biomarker in various thrombotic conditions.