Related Experiment Video
Updated: Sep 12, 2025
In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
Published on: June 17, 2019
in vivo Reprogramming of NG2 Glia Improves Bladder Function After Spinal Cord Injury
Wenjiao Tai1,2, Junkui Shang3,2, Peiqi Zhao1,2
1Department of Molecular Biology, Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
None:
Neurogenic bladder is a debilitating consequence of spinal cord injury (SCI), with few treatment options that restore voluntary voiding. Here, we show that SOX2-mediated in vivo reprogramming of NG2 glia improves bladder function in a clinically relevant mouse model of contusive SCI. NG2 glia reprogramming induces adult neurogenesis, reduces glial scarring, and significantly improves urinary performance, as measured by voiding assays and conscious cystometry. Functional recovery correlates positively with neurogenesis and negatively with glial scarring. These findings demonstrate that SOX2-mediated glial reprogramming promotes autonomic repair and offers a regenerative strategy for neurogenic bladder after SCI.
