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Updated: Sep 12, 2025

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Basal Cell-Contact Dynamics Influence Tissue Packing in a Proliferating Mammalian Epithelium.

Subramanian P Ramanathan1,2, Tanmoy Sarkar3, Matej Krajnc3

  • 1Department of Genetics, Cell Biology & Anatomy, University of Nebraska Medical Center.

Biorxiv : the Preprint Server for Biology
|August 6, 2025
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Summary

Epithelial cell division involves dynamic shape changes that rearrange cell contacts. These mitotic dynamics, including cell volume changes and daughter cell reinsertion, are crucial for tissue packing and development.

Keywords:
Epithelial organizationbasal rosettescell divisioncell neighbor exchangescell packingcell shapecell-contact dynamicsintestinal organoidsvertex model simulations

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biophysics

Background:

  • Epithelial tissue morphology depends on cell shape, position, and proliferation.
  • The influence of cell shape changes during mitosis on tissue packing, especially basal cell contacts, is not fully understood.

Purpose of the Study:

  • To investigate the dynamic rearrangements of basal cell contacts during epithelial cell division.
  • To elucidate the role of mitotic cell shape transformations in epithelial tissue packing and morphogenesis.

Main Methods:

  • Utilized an in silico vertex model to simulate epithelial mitotic rounding and basal contact dynamics.
  • Employed a mammalian intestinal organoid model to experimentally validate in silico predictions.
  • Used pharmacological inhibition of mitotic progression to dissect the contribution of specific mitotic events.

Main Results:

  • In silico modeling predicted a sequence of basal contact rearrangements during mitosis, including volume diminution, rosette formation, and daughter cell reinsertion.
  • Experimental validation in intestinal organoids confirmed similar basal cell-contact dynamics as predicted.
  • Pharmacological inhibition showed that basal cell body diminution and daughter cell reinsertion independently drive distinct contact rearrangements.

Conclusions:

  • Epithelial mitotic rounding necessitates dynamic basal contact rearrangements.
  • Basal cell division dynamics play a significant, previously underappreciated topological role in epithelial packing and morphogenesis.