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Salidroside alleviates cardiomyocyte senescence by activating the AMPK-mediated signaling pathway
Lan Zhang1, Yawei Liu1, Zhijuan Hua2
1Department of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Hebei University, Baoding, China.
Insights
Salidroside, a compound from Rhodiola rosea, effectively reduces aging in heart cells (cardiomyocytes) by repairing DNA damage. This natural compound shows promise for preventing age-related cardiovascular diseases.
Area of Science:
- Cardiology
- Gerontology
- Pharmacology
Background:
- Cardiovascular aging significantly increases the risk of cardiovascular diseases (CVDs), a leading cause of mortality in older adults.
- There is a critical need for novel therapeutic strategies and bioactive molecules to mitigate cardiovascular aging and associated pathologies.
Purpose of the Study:
- To investigate the anti-aging effects of salidroside, a bioactive compound found in Rhodiola rosea extract, on cardiomyocytes.
- To evaluate salidroside's potential in preventing and treating age-related cardiovascular dysfunction.
Main Methods:
- An in vitro model of cardiomyocyte senescence was established using D-galactose and hydrogen peroxide induction.
- Techniques including Western blot, indirect immunofluorescence, ELISA, and flow cytometry were utilized to assess senescence markers and cellular damage.
- Sa-β-gal staining was performed to quantify senescent cells.
Main Results:
- Salidroside significantly inhibited cardiomyocyte senescence, as evidenced by reduced Sa-β-gal activity and lower expression of senescence markers (p16, p21, p53).
- The treatment decreased levels of senescence-associated inflammation and oxidative stress within the cardiomyocytes.
- Mechanistic studies revealed that salidroside promotes anti-aging effects through the repair of DNA damage.
Conclusions:
- Salidroside exhibits potent anti-aging properties against cardiomyocyte senescence.
- This compound demonstrates potential as a functional food ingredient for combating cardiovascular aging.
- Salidroside offers a promising therapeutic avenue for preventing age-related cardiovascular diseases.
Abstract:
Cardiovascular aging is a key contributor to cardiovascular diseases (CVDs). As individuals age, the frequency and severity of cardiovascular events rise, establishing CVDs as a primary cause of death in older adults. Therefore, the development and exploration of drugs or bioactive molecules that can effectively prevent cardiovascular aging and related diseases are urgently needed. This study evaluated the effects of salidroside, a key component of Rhodiola rosea extract, on cardiomyocyte senescence. We established an in vitro cardiomyocyte senescence model using D-gal/H2O2 induction. A series of experimental techniques, including Western blot, indirect immunofluorescence, ELISA, and flow cytometry, were employed. The findings indicated that salidroside markedly reduced cardiomyocyte senescence in this model, as shown by Sa-β-gal staining and the evaluation of senescence-associated markers such as p16, p21, and p53. Additionally, salidroside reduced senescence-associated inflammation and oxidative stress levels. Additional mechanistic research demonstrated that salidroside promotes anti-aging by repairing DNA damage. In summary, this study systematically evaluated the potential biological activity of salidroside in combating cardiomyocyte aging and demonstrated its promising anti-aging effects. These findings suggest that salidroside could serve as a functional food ingredient with anti-aging properties, offering new strategies for preventing cardiovascular aging and related diseases.
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