Related Experiment Video
Updated: Sep 12, 2025
09:59
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
Published on: June 13, 2025
530
From Vision to Reality: A Perspective on the Innovative and Collaborative Forces That Evolved Proximity Labeling
Tess C Branon1, Wei Qin2, Namrata D Udeshi3
1Proteomic & Genomic Technologies, Genentech, Inc, South San Francisco, California, USA.
Molecular & Cellular Proteomics : MCP
|August 6, 2025
Summary
Proximity labeling, using APEX and TurboID, offers high-resolution insights into living systems. This perspective details the development and application of these powerful techniques for biological research.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Proximity labeling is crucial for studying biological systems with high spatial and temporal resolution.
- Mass spectrometry is a key technology used in conjunction with proximity labeling.
Purpose of the Study:
- To provide a historical overview of the development of APEX and TurboID proximity labeling technologies.
- To offer guidance on executing successful proximity labeling experiments.
- To discuss future directions in proximity labeling research.
Main Methods:
- The perspective focuses on APEX (Avidin-Biotin Proximity Extension Assay) and TurboID proximity labeling methods.
- Mass spectrometry is implicitly used for analyzing labeling products.
Main Results:
- The development of APEX and TurboID technologies resulted from innovation and collaboration.
- Guidance for successful experimental execution is provided.
- Future developments in the field are discussed.
Conclusions:
- APEX and TurboID are vital tools for advancing biological research.
- Collaboration and innovation are key to developing new biological technologies.
- The field of proximity labeling holds significant promise for future discoveries.
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