Related Experiment Video

Updated: Sep 12, 2025

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

15.1K

Post-translational modifications of Stat3: The state of the art

Jiaxu Chen1, Caiyun Mao1, Ning Han1

  • 1Department of Pharmacology, Heilongjiang University of Chinese Medicine, Harbin, China.

Cellular Signalling
|August 6, 2025
PubMed

Insights

Signal transducer and activator of transcription 3 (Stat3) is vital for cell functions and disease development. Its activity is precisely controlled by various post-translational modifications (PTMs), offering new therapeutic targets.

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Biochemistry

Background:

  • Signal transducer and activator of transcription 3 (Stat3) is a key transcription factor involved in cell proliferation, differentiation, and development.
  • Dysregulated Stat3 activity is implicated in the pathogenesis of cardiovascular diseases and various cancers, including breast, pancreatic, and renal cell carcinoma.

Purpose of the Study:

  • To review the diverse post-translational modifications (PTMs) that regulate Signal transducer and activator of transcription 3 (Stat3) function.
  • To provide a comprehensive analysis of the regulatory mechanisms governing Stat3 within cellular signaling networks.
  • To highlight the potential of Stat3-targeting pathways for novel therapeutic strategies.

Main Methods:

  • Literature review of existing research on Stat3.
  • Analysis of various post-translational modifications (PTMs) affecting Stat3.
  • Exploration of Stat3's role in disease pathogenesis and cellular signaling.

Main Results:

  • Stat3 function is modulated by multiple PTMs, including phosphorylation, sulfenylation, acetylation, sulfhydrylation, and SUMOylation.
  • These modifications induce structural changes in Stat3, altering its transcriptional activity and DNA-binding stability.
  • Pathophysiological signals trigger PTMs, leading to distinct gene expression programs in different cell types.

Conclusions:

  • Post-translational modifications are critical regulators of Signal transducer and activator of transcription 3 (Stat3) activity.
  • Understanding these regulatory mechanisms provides insights into Stat3's role in disease.
  • Targeting Stat3 PTMs presents a promising avenue for developing innovative therapeutic strategies for cancer and cardiovascular diseases.
Keywords:
AcetylationMethylationPhosphorylationPost-translational modificationsStat3SulfenylationSulfhydrylation

More Related Videos

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.6K
Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells13:20

Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Published on: July 29, 2010

28.8K

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.2K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.2K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.0K
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
51.2K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.6K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.8K