Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Direct-Acting Cholinergic Agonists: Therapeutic Uses01:11

Direct-Acting Cholinergic Agonists: Therapeutic Uses

885
Direct-acting cholinergic agonists have many therapeutic uses in various medical fields. Choline esters, including acetylcholine, have limited clinical utility due to their non-selectivity and short duration of action. Still, acetylcholine and carbachol are applied topically during ophthalmologic surgery to induce miosis. Pilocarpine, a muscarinic and ganglionic stimulator, effectively treats open-angle glaucoma and alleviates xerostomia and dry mouth caused by radiotherapy or Sjögren...
885
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies01:27

Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies

2.6K
Assessing and diagnosing Chronic Obstructive Pulmonary Disease (COPD) involves a detailed approach that includes a comprehensive review of medical history, physical examination, and a variety of diagnostic tests. This thorough evaluation is essential to ensure an accurate diagnosis and guide effective management strategies.
Medical History
2.6K
Myasthenia Gravis: Diagnostic Tests01:15

Myasthenia Gravis: Diagnostic Tests

1.3K
Myasthenia gravis is an autoimmune condition affecting neuromuscular transmission, causing generalized weakness in skeletal muscles. Initial diagnoses rely on patients' signs, symptoms, and medical history. The challenge lies in distinguishing myasthenia from other muscular dystrophies. An important diagnostic feature is the significant improvement of symptoms after administering anticholinesterase inhibitors.
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
1.3K
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

3.1K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
3.1K
Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship01:22

Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship

1.2K
Cholinergic agonists or cholinomimetics mimic the action of acetylcholine to stimulate the parasympathetic nervous system. They are categorized into direct-acting and indirect-acting agents. The direct-acting cholinergic drugs induce the parasympathetic response by directly binding to the muscarinic or nicotine receptors. In comparison, the indirect-acting cholinergic drugs prevent acetylcholine hydrolysis, indirectly contributing to the extended parasympathetic response.
The direct-acting...
1.2K
Chronic Obstructive Pulmonary Disease-I: Introduction01:20

Chronic Obstructive Pulmonary Disease-I: Introduction

2.9K
Chronic Obstructive Pulmonary Disease (COPD) is a long-lasting respiratory condition requiring continuous attention and care. It is a progressive lung disease that leads to breathing challenges due to airflow obstruction. It manifests as persistent respiratory symptoms and restricted airflow resulting from abnormalities in the airways and alveoli, usually due to long-term exposure to harmful particles or gases. COPD mainly consists of two primary conditions: emphysema and chronic bronchitis.
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FeNO as a Treatable Trait in Chronic Cough: Insights from the PROCOUGH Study.

Lung·2026
Same author

Asthma diagnosis in adults and children: challenges, future directions, and a call to action.

The Lancet. Respiratory medicine·2026
Same author

The CoughRetrain Program: Restoring Control Through a Non-pharmacological Intervention.

Lung·2026
Same author

Dupilumab in COPD: A Pooled Analysis of Emergency Department Visits, Hospital Admissions, and Systemic Corticosteroid Use.

American journal of respiratory and critical care medicine·2026
Same author

Moving Beyond Responders: A Post Hoc Analysis Identifying Criteria for Chronic Cough Control.

Chest·2026
Same author

Reply to: Is the role of eosinophils in refractory chronic cough truly dismissed? Mepolizumab reduced blood and sputum eosinophils, but not eosinophil-derived neurotoxin.

The European respiratory journal·2026

Related Experiment Video

Updated: Sep 12, 2025

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography
08:58

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography

Published on: July 10, 2018

43.3K

Methacholine Challenge: Physiology, Methodology, and Clinical Interpretation.

Imran Satia1, Mark Inman2, Beth E Davis3

  • 1Division of Respirology, Department of Medicine, McMaster University, Hamilton, Ontario L8S 4L8, Canada; Firestone Institute for Respiratory Health, St Joseph's Healthcare, Hamilton, Ontario L8N 4A6, Canada.

Clinics in Chest Medicine
|August 6, 2025
PubMed
Summary

The methacholine challenge test (MCT) assesses airway hyperresponsiveness (AHR) in asthma diagnosis. This review covers MCT history, mechanisms, and future improvements for AHR assessment.

Keywords:
Airways diseaseAsthmaBronchial challengeMethacholine

More Related Videos

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique
13:10

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

Published on: May 15, 2013

57.3K
Murine Model of Allergen Induced Asthma
08:05

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

40.5K

Related Experiment Videos

Last Updated: Sep 12, 2025

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography
08:58

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography

Published on: July 10, 2018

43.3K
Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique
13:10

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

Published on: May 15, 2013

57.3K
Murine Model of Allergen Induced Asthma
08:05

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

40.5K

Area of Science:

  • Pulmonary Medicine
  • Respiratory Physiology

Background:

  • Airway hyperresponsiveness (AHR) is a key feature of asthma.
  • The methacholine challenge test (MCT) is a standard method for evaluating AHR.
  • Understanding AHR mechanisms is crucial for asthma management.

Purpose of the Study:

  • To provide a comprehensive review of the methacholine challenge test (MCT).
  • To discuss the historical development, physiological basis, and clinical applications of MCT.
  • To explore advancements, interpretation factors, limitations, and future directions for MCT.

Main Methods:

  • Literature review of historical and contemporary studies on MCT.
  • Analysis of physiological mechanisms underlying AHR and bronchoconstriction.
  • Evaluation of MCT methodology, diagnostic utility, and influencing factors.

Main Results:

  • The MCT has evolved significantly since its inception.
  • MCT plays a vital role in diagnosing asthma by quantifying AHR.
  • Factors such as medication, recent infections, and comorbidities can affect MCT results.

Conclusions:

  • The MCT remains a valuable tool for assessing AHR and aiding asthma diagnosis.
  • Further research is needed to refine MCT protocols and interpretation guidelines.
  • Optimizing MCT can enhance its utility in clinical practice and research.