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Human prolactin heterogeneity in pituitary effluent blood
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|November 1, 1985
Summary
This study reveals that during secretory pulses, the pituitary gland releases more small prolactin (PRL) forms. Larger and intermediate PRL forms contribute less variably to increased PRL levels.
Area of Science:
- Endocrinology
- Molecular Endocrinology
- Pituitary Gland Physiology
Background:
- Prolactin (PRL) exists in various molecular forms, including small (peak III), intermediate (peak II), and large (peak I) PRL.
- Understanding PRL heterogeneity is crucial for interpreting hyperprolactinemia and pituitary function.
Purpose of the Study:
- To investigate the molecular forms of prolactin secreted during a secretory pulse.
- To analyze PRL heterogeneity in plasma from the inferior petrosal vein, near the anterior pituitary.
Main Methods:
- Column chromatography was employed to separate and quantify different PRL forms.
- Plasma samples were collected from hyperprolactinemic subjects during inferior petrosal vein venography.
Main Results:
- Increased PRL concentration during venography was associated with a higher percentage and amount of small PRL (peak III).
- The proportion of intermediate PRL (peak II) decreased, while large PRL (peak I) remained relatively constant or increased slightly.
- One patient showed a distinct pattern with predominantly large PRL initially, followed by a significant increase in small PRL as total PRL rose.
Conclusions:
- The primary source of increased PRL during a secretory pulse is the small PRL form.
- The contributions of large and intermediate PRL forms to PRL pulses are modest and exhibit variability among individuals.