Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clocked stepping of an artificial protein walker along a DNA track.

Nature nanotechnology·2026
Same author

Role of Irradiance in Light-Activated In<sub>2</sub>O<sub>3</sub> Gas Sensors: Why More Light Is Not Always Better.

ACS sensors·2026
Same author

The Impact of Polyethylene Glycol Lipid Anchors on the Physicochemical Properties, Protein Corona, Function, and Biodistribution of Lipid Nanoparticles.

ACS nano·2026
Same author

Effects of Serum Incubation on Lipid Nanoparticle PEG Shedding, mRNA Retention, and Membrane Interactions.

ACS applied materials & interfaces·2025
Same author

Deep Sub-Wavelength 3D Imaging Using a Single Nanowire Detector.

Nano letters·2025
Same author

Quantitative Detection of Biological Nanoparticles Using Twilight Off-Axis Holographic Microscopy: Insights on Complex Formation between PEGylated Gold Nanoparticles and Lipid Vesicles.

The journal of physical chemistry. B·2025

Related Experiment Video

Updated: Jun 13, 2026

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

22.5K

Image analysis optimization for nanowire-based optical detection of molecules.

Rubina Davtyan1,2, Nicklas Anttu3, Julia Valderas-Gutiérrez1,2

  • 1Division of Solid State Physics, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.

Nanophotonics (Berlin, Germany)
|August 7, 2025
PubMed
Summary
This summary is machine-generated.

Semiconductor nanowires boost fluorescence detection sensitivity. Digital detection with single-emitter localization significantly improves optical biosensing dynamic range and accuracy for molecular assays.

Keywords:
TIRFbiosensingepifluorescencefluorescence microscopyimage analysisnanowire

More Related Videos

Implementation of a Reference Interferometer for Nanodetection
16:11

Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

9.5K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.2K

Related Experiment Videos

Last Updated: Jun 13, 2026

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

22.5K
Implementation of a Reference Interferometer for Nanodetection
16:11

Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

9.5K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.2K

Area of Science:

  • Nanotechnology
  • Optical Biosensing
  • Biophysics

Background:

  • Semiconductor nanowires enhance fluorescence signals in optical biosensing.
  • Improving detection limits is crucial for sensitive molecular assays.

Purpose of the Study:

  • To enhance optical biosensing sensitivity using "digital" detection with nanowires.
  • To evaluate the impact of single-emitter localization on detection sensitivity and dynamic range.
  • To develop a systematic analysis pipeline for digital nanowire detection.

Main Methods:

  • Employing vertically aligned nanowires for "digital" detection.
  • Utilizing single-emitter localization methods with bright-field microscopy.
  • Performing streptavidin-biotin assays and time-resolved titration experiments.
  • Validating the analysis framework with simulated data based on Maxwell's equations.

Main Results:

  • Single-emitter localization expanded the dynamic range to five orders of magnitude (10 µM to 10 nM).
  • Achieved a two to three orders of magnitude improvement in detection sensitivity compared to non-localization methods.
  • Nanowires demonstrated higher sensitivity at low concentrations than total internal reflection fluorescence microscopy.
  • Higher protein capture rate and intensity per binding event contributed to enhanced nanowire sensitivity.

Conclusions:

  • Digital detection with nanowires and single-emitter localization offers superior sensitivity and dynamic range for optical biosensing.
  • The developed analysis pipeline effectively harnesses digital detection capabilities.
  • Nanowire-based biosensing shows significant promise for both endpoint and time-resolved applications.
  • This approach provides a substantial improvement over conventional fluorescence detection methods.