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Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights.

Hua Han1, Bin Han2, Yongjin Peng3,4

  • 13rd Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.

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Summary

This study introduces JFT, a fluorescence probe detecting sulfite and monitoring viscosity using Fluorescence Resonance Energy Transfer (FRET) and Twisted Intramolecular Charge Transfer (TICT) mechanisms. JFT

Keywords:
fluorescent probeintracellular viscositymultifunctionalsulfitetheoretical investigation

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Area of Science:

  • Chemical sensing
  • Fluorescence spectroscopy
  • Molecular probes

Background:

  • Fluorescence probes are crucial for detecting analytes and monitoring cellular environments.
  • Developing multifunctional probes with high sensitivity and specificity remains a challenge.
  • Understanding probe mechanisms like FRET and TICT is key for rational design.

Purpose of the Study:

  • To develop and characterize a novel multifunctional fluorescence probe, JFT.
  • To investigate JFT's ability to detect sulfite and measure intracellular viscosity.
  • To elucidate the underlying Fluorescence Resonance Energy Transfer (FRET) and Twisted Intramolecular Charge Transfer (TICT) mechanisms.

Main Methods:

  • Synthesis and characterization of the JFT probe.
  • Spectroscopic studies (fluorescence emission/excitation) in response to sulfite.
  • Viscosity-dependent fluorescence measurements.
  • Theoretical calculations using Time-Dependent Density Functional Theory (TDDFT).

Main Results:

  • JFT successfully detected sulfite by altering its fluorescence properties, indicating a turn-off of FRET.
  • JFT demonstrated sensitivity to changes in viscosity, correlating with altered non-radiative decay pathways.
  • TDDFT calculations confirmed electron distribution changes upon sulfite interaction, aligning with experimental observations.

Conclusions:

  • JFT is a versatile fluorescence probe capable of dual-mode sensing (sulfite detection and viscosity monitoring).
  • The study provides insights into the interplay of FRET and TICT mechanisms in probe design.
  • Findings offer theoretical guidance for creating advanced, efficient fluorescence probes for biological applications.