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Updated: Sep 12, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Nondestructive DNA extraction from specimens and bulk samples preserved in DESS solution for DNA barcoding.

Eri Ogiso-Tanaka¹, Minako Abe Ito², Daisuke Shimada^1,3

¹Center for Molecular Biodiversity Research, National Museum of Nature and Science, Tsukuba, Ibaraki, Japan.

|August 7, 2025

Summary

This study introduces a nondestructive DNA extraction method using DESS preservation solution supernatant. This technique preserves specimen morphology and DNA, enabling long-term barcoding of nematodes and environmental samples.

Keywords:

18S COI DNA barcoding diatom nGS nanopore nematode nondestructive DNA extraction specimens

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Area of Science:

Molecular Biology
Genetics
Ecology

Background:

DNA barcoding is vital for species identification.
Traditional methods often damage or destroy small specimens.
Nondestructive DNA extraction is needed to preserve morphology.

Purpose of the Study:

To develop a nondestructive DNA extraction protocol.
To preserve both DNA and specimen morphology.
To enable long-term storage and barcoding of small organisms and environmental samples.

Main Methods:

Utilizing the supernatant of DESS (20% DMSO, 250 mM EDTA, saturated NaCl) preservation solution.
Applying the protocol to nematodes preserved for 10 years.
Adapting the method for bulk environmental samples (sediment, seagrass).

Main Results:

Successful nondestructive DNA barcoding of nematodes.
Preservation of morphological characteristics alongside DNA.
Demonstrated applicability to environmental samples for meiofauna and diatom barcoding.

Conclusions:

The DESS supernatant method offers a viable solution for nondestructive DNA extraction.
This protocol facilitates long-term preservation and barcoding of diverse biological samples.
It is applicable to both individual specimens and bulk environmental collections.