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Single Cell Sequencing Reveals Cellular Heterogeneity in Mouse Dorsal Root Ganglion
Yiting Chen1,2, Yuying Wang1,2, Yucong Zhu1,2
1The Second Affiliated Hospital, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Guangzhou Medical University, Guangzhou, China.
Journal of Neurochemistry
|August 7, 2025
Summary
This study reveals the diverse cell types within the mouse dorsal root ganglion (DRG) and their functions. Understanding DRG cellular heterogeneity offers new insights into sensory physiology.
Area of Science:
- Neuroscience
- Cell Biology
- Genomics
Background:
- The dorsal root ganglion (DRG) is vital for sensory information processing.
- The specific roles of diverse DRG cell types are not well understood.
Purpose of the Study:
- To characterize the cellular landscape and functional heterogeneity of the mouse DRG.
- To explore cell-cell communication networks and evolutionary trajectories within the DRG.
Main Methods:
- Analysis of single-cell RNA sequencing data from mouse DRG (GSE155622).
- Cell type identification, functional enrichment analysis (QuSAGE, GO, KEGG), and pseudo-temporal analysis.
- Validation using RNAscope in situ hybridization and ISH-IHC double staining.
Main Results:
- Identification of multiple cell types including neurons, immune cells, fibroblasts, glial cells, and vascular cells.
- Functional enrichment analysis revealed distinct roles for each cell subtype.
- Construction of a comprehensive cellular communication network and determination of cell evolutionary paths.
- Validation of marker and itch-related gene expression in mouse DRG tissues.
Conclusions:
- Mouse dorsal root ganglion exhibits significant cellular heterogeneity.
- These findings provide novel insights into DRG physiology and sensory mechanisms.
