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Updated: Sep 12, 2025

Culture and Transfection of Zebrafish Primary Cells
Published on: August 17, 2018
Protocol for the isolation, culture, and transfection of squid primary cells
Yoonji Kim1, Hailey M Tanner2, Joshua J C Rosenthal3
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ 08544, USA.
Abstract:
We present a protocol for the isolation, culture, and transfection of squid primary cells from various tissues and ages by including steps for squid dissection, cell isolation, maintenance in culture, and expression of exogenous genes. We also highlight life-stage tractability in Euprymna berryi squids for future aging studies. This protocol enables molecular- and cellular-level investigations into processes specific to squids, including their complex behaviors, rapid color change mechanisms, and RNA editing capabilities, with broader implications in basic cell physiology.
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