Studies on N1-DNCP-N6-lactobionoyl-1,6-hexanediamine--a distinctive monovalent anaphylactogen

N1-DNCP-N6-lactobionoyl-1,6,hexanediamine, which is a potent anaphylactogen when i.v. injected into anti-DNCP sensitized guinea pigs, was shown to be also anaphylactogenic when administered intradermally into guinea pigs passively sensitized by i.v. injection of anti-DNCP antiserum. The compound, which is monohaptenic conjugate with the DNCP group as haptenic part and the carbohydrate moiety as auxiliary group, was found to be unable to precipitate high-titered anti-DNCP antisera. This precludes formation of simple, functionally oligovalent associates. Since many monohaptenic anaphylactogens with various hydrocarbon chains as auxiliary groups do not induce anaphylaxis when given intradermally, the anaphylaxis, demonstrated after intradermal antigen application, allows a biological distinction between the lactobionoyl and similar conjugates, and the hydrocarbon-bearing anaphylactogens.