Bacillus subtilis morphology and bentonite colloids govern Eu(III) transport in quartz sand: Mechanisms and machine...

Microorganisms critically regulate radionuclide migration through diverse biological mechanisms. Bacillus subtilis (B. subtilis) exhibits strong adsorption capacity, immobilizing radionuclides and limiting their mobility. By contrast, biological colloids predominantly enhance transport via complexation and mobilization. This study systematically investigates the influence of B. subtilis cells, spores, extracellular polymeric substances (EPS), and bentonite colloids (BC) on Eu(III) transport in quartz sand. Integrating advanced microscopy, spectroscopy, and machine learning, we reveal that Eu(III) mobility is governed by intricate interactions among bacterial activity, spores, EPS, and BC. Notably, inactivated B. subtilis colloids exhibited higher mobility yet more pronounced Eu(III) transport inhibition compared to live cells. Under neutral pH/low ionic strength conditions, B. subtilis cells and spores enhanced Eu(III) transport by 63 %, whereas acidic pH/high ionic strength conditions reduced transport by 30 % for cells, with spores maintaining elevated mobility. EPS removal increased both bacterial mobility and Eu(III) transport efficiency by 20 %, particularly under acidic conditions. While B. subtilis elevated Eu(III) mobility by 63 %, BC attenuated this effect by 13 %, primarily through the formation of BC-B. subtilis-Eu(III) ternary pseudo-colloids. Machine learning identified BC concentration and pH as key governing factors. These findings provide critical insights into microbial-mediated radionuclide transport and advance predictive risk assessment models for high-level radioactive waste disposal.