Related Experiment Video
Updated: Sep 12, 2025
Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
Published on: March 7, 2013
Actual versus ideal donor body weight in planning bone marrow harvest
Rui Murahashi1, Shin-Ichiro Fujiwara1, Daisuke Minakata2
1Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan; Division of Cell Transplantation and Transfusion, Jichi Medical University, Tochigi, Japan.
Background:
Donor selection and harvest volume calculation typically use actual body weight (ABW). However, in obese donors, increased ABW mainly reflects adipose tissue rather than hematopoietic stem cell (HSC)-rich bone marrow. This study aimed to investigate whether ideal body weight (IBW) is a better indicator than ABW for estimating the required HSC yield.
Study Design And Methods:
This study included 157 donors (106 men and 51 women) with a body mass index of ≥ 22. An analysis of the correlation between harvested HSCs and harvested volume per ABW and IBW was performed. Another analysis of the correlation between the decrease in hemoglobin levels and the harvested volume per ABW or IBW was performed.
Results:
No significant differences were observed in the correlations between total nucleated cell counts and the harvested volume per ABW or IBW, between CD34+ HSCs and the harvested volume per ABW or IBW, and between the decrease in hemoglobin levels and the harvested volume per ABW or IBW in all donors and obese donors.
Conclusions:
These results showed no significant differences between IBW and ABW as indicators for estimating the required HSC yield. Additionally, the results showed that harvesting per ABW was not excessive.
Related Concept Videos
01:22Bone Marrow Sampling and Transplants
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
01:28Kidney Transplant I: Introduction