Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simple liver perfusion model for NMR spectroscopic analysis.

M E Clouse, R G Lee

    Investigative Radiology
    |November 1, 1985
    PubMed
    Summary

    We developed a novel perfused mouse liver model for nuclear magnetic resonance spectroscopy studies. This technique allows real-time monitoring of liver metabolism and injury markers during ischemia and anoxia.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Heparin-binding EGF-like growth factor (HB-EGF) antisense oligonucleotide protected against hyperlipidemia-associated atherosclerosis.

    Nutrition, metabolism, and cardiovascular diseases : NMCD·2019
    Same author

    Targeted delivery of antisense oligonucleotides to pancreatic β-cells.

    Science advances·2018
    Same author

    Quantitative coronary arterial stenosis assessment by multidetector CT and invasive coronary angiography for identifying patients with myocardial perfusion abnormalities.

    Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2012
    Same author

    PCBs in U.K. Urban Air.

    Environmental science & technology·2012
    Same author

    Accuracy of dual-source CT in the characterisation of non-calcified plaque: use of a colour-coded analysis compared with virtual histology intravascular ultrasound.

    The British journal of radiology·2009
    Same author

    Characterization of coronary atherosclerosis by dual-source computed tomography and HU-based color mapping: a pilot study.

    European radiology·2008

    Area of Science:

    • Physiology
    • Biochemistry
    • Medical Imaging

    Background:

    • Studying liver physiology and metabolic changes requires advanced in vivo techniques.
    • Nuclear Magnetic Resonance (NMR) spectroscopy offers detailed metabolic insights.
    • A specialized model is needed to combine NMR with perfusate analysis of the liver.

    Purpose of the Study:

    • To develop and validate a novel perfused mouse liver model for vertical-bore NMR spectroscopy.
    • To enable simultaneous assessment of liver metabolism and perfusate composition.
    • To investigate the effects of ischemia and anoxia on liver function.

    Main Methods:

    • Developed a technique for perfusing a mouse liver within a vertical-bore NMR spectrometer.
    • Cannulated the portal vein for perfusate inflow and utilized an outlet for perfusate sampling.
    • Utilized oxygenated Krebs-Henseleit buffer for perfusion and P-31 NMR spectroscopy.
    • Correlated NMR data with biochemical measurements of liver enzyme release.

    Main Results:

    • Achieved excellent P-31 NMR spectra of the mouse liver.
    • Demonstrated stable liver energy charge (ATP/Pi) under perfusion.
    • Successfully observed and characterized the effects of ischemia and anoxia on liver metabolism.
    • Correlated spectral changes with liver enzyme release, indicating cellular injury.

    Conclusions:

    • The developed perfused mouse liver model is effective for NMR spectroscopy.
    • This model allows real-time monitoring of liver metabolic status and injury.
    • It provides a valuable tool for studying liver pathophysiology, including ischemia and anoxia.

    Related Experiment Videos