Jove
Visualize
Contact Us

Related Concept Videos

PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of a patient decision aid for intraocular lens selection in cataract surgery.

Patient education and counseling·2026
Same author

Mediating effect of coping styles between self-efficacy and diabetes distress among Chinese patients with diabetic retinopathy: a cross-sectional study.

BMJ open·2025
Same author

The Effect of a Patient Decision Aid Developed for Chinese Primary Angle Closure Patients: A Randomized Control Trial.

Patient preference and adherence·2025
Same author

Gold Nanoparticles Carrying Mannose-Binding Lectin and Inflammatory Cytokine Antibodies Improve Sepsis Survival by Modulating Immunity and Reducing Pathogens.

ACS applied materials & interfaces·2025
Same author

Illness Uncertainty, Burden, and Capacity Among Caregivers of Children With Chronic Uveitis: A Stress and Coping Theory Perspective.

American journal of ophthalmology·2025
Same author

Experiences of burden and needs in caregivers with pediatric noninfectious uveitis: a qualitative study.

BMC nursing·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 3, 2026

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

11.0K

Digital PCR in prenatal diagnosis.

Ling Zhang1, Lu Chen1, Yali Yu1

  • 1Department of Gynecology, Northwest Women and Children's Hospital, Xi'an, Shaanxi 710061, China.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|August 7, 2025
PubMed
Summary

Digital PCR (dPCR) offers high sensitivity for non-invasive prenatal testing (NIPT), enabling precise detection of fetal DNA. Further advancements in dPCR technology are crucial for broader clinical adoption in prenatal diagnostics.

Keywords:
Chromosome aneuploidyDigital PCRMonogenic diseasesNIPTPGT

More Related Videos

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

9.5K
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.2K

Related Experiment Videos

Last Updated: Jul 3, 2026

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

11.0K
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

9.5K
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Non-invasive prenatal testing (NIPT) relies on detecting low-abundance fetal DNA.
  • Digital PCR (dPCR) offers superior sensitivity and absolute quantification for NIPT applications.

Purpose of the Study:

  • To review the applications of dPCR in NIPT.
  • To highlight dPCR's advantages in identifying fetal DNA and genetic variations.
  • To discuss areas for improvement and compare dPCR platforms for prenatal diagnostics.

Main Methods:

  • Literature review of dPCR applications in NIPT.
  • Comparison of different dPCR platforms' principles and features.
  • Analysis of dPCR's role in detecting various targets and diseases.

Main Results:

  • dPCR excels in detecting low-abundance fetal DNA and complex genetic variations in NIPT.
  • Various dPCR platforms show promise for prenatal diagnostics.
  • Key areas for improvement include sensitivity, cost, and standardization.

Conclusions:

  • dPCR is a powerful tool for NIPT, with significant potential for advancing prenatal diagnostics.
  • Continued research and development are essential to optimize dPCR for clinical use.
  • Standardization and cost-effectiveness will drive wider adoption of dPCR in NIPT.