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Published on: February 12, 2020
Study on the mechanism of LNC004268 regulating sheep myoblast proliferation and differentiation through hnRNPK-CNOT2
Yize Song1, Yufang Liu2, Runqing Chi2
1College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Abstract:
The long non-coding RNAs (lncRNAs) can bind to transcription factors or RNA-binding proteins to play important regulatory roles in muscle growth and development. This study investigated the functional role of the LNC_004268 in sheep myoblast proliferation and differentiation, as well as its interaction with the RNA-binding protein hnRNPK and the downstream target gene CNOT2. LNC_004268 shows higher expression in the longissimus dorsi muscle of Small-tailed Han sheep (STH) compared to Sunite sheep (SNT) and is primarily localized in the nucleus. Functionally, LNC_004268 inhibits myoblast proliferation while promoting differentiation and myotube formation. Mechanistically, LNC_004268 binds hnRNPK, stabilizing CNOT2 mRNA. Actinomycin D (ACD) assays confirmed that LNC_004268 or hnRNPK overexpression delays CNOT2 mRNA decay, with combined expression further enhancing stability. Notably, CNOT2 functionally recapitulates LNC_004268's effects on myogenesis. Collectively, our study demonstrates that LNC_004268 regulates myoblast proliferation and differentiation by stabilizing CNOT2 mRNA via hnRNPK interaction, advancing understanding of lncRNA mediated muscle development regulation.
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