Integrated transcriptomics and metabolomics revealed improvement of the nervous system in old rats by aspirin eugenol...

Elderly groups usually have an inflammatory response in their bodies, and neuroinflammation is the main pathogenic mechanism of neurological diseases. Pharmacological and pharmacodynamic experiments showed that aspirin eugenol ester (AEE) had good anti-inflammatory, anti-oxidant and anti-hyperlipidemic effects. However, whether AEE can prevent neurological diseases in the old companion animal has not been explored. In this study, we selected adolescent rats (6 weeks old) and old rats (18 months old) as the experimental animals for two weeks. The transcriptomics and metabolomics methods were used to explore the effects of AEE on the hippocampal region in the brains of old rats. The related indexes were detected. AEE significantly reduced the levels of pro-inflammatory cytokines and oxidative stress in lipopolysaccharide (LPS) induced microglia of rats. AEE significantly increased the duration of swimming observed in the forced swimming test for older rats, but did not have a significant effect between adolescent rats. Hematoxylin and eosin staining (HE) showed that AEE had no significant effects on the kidney and gastrointestinal tract and could improve liver function in old rats. AEE improved genes and metabolites in the hippocampal region of the old rat brain. Comprehensive analysis of transcriptomics and metabolomics showed that the gene Chacna3 was correlated positively with metabolic phosphatidylethanolamine (PE) and phosphatidylcholine (PC), and the genes Tnfsf10 and Cacna1f were correlated positively with metabolic PE and PC and negatively with eicosapentaenoic acid (Epa). AEE could play positive effects on preventing neurological diseases in old companion animal.