Related Experiment Video
Updated: May 7, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
Published on: December 15, 2012
Altered cell length in deletion mutants of the different divIVA genes in Rhodococcus erythropolis PR4
Shabnam Parwin1, Preeti Srivastava2
1Department of Biochemical Engineering & Biotechnology, Indian Institute of Technology, Delhi, New Delhi, India.
Abstract:
Unlike other Actinobacteria, Rhodococcus erythropolis PR4 contains three different copies of divIVA genes. The three divIVA genes show about 22-38% similarity with each other. Deletion mutants of the divIVA genes resulted in altered morphology: elongated cells in △divIVA-2 and short rod-shaped cells in △divIVA-1 and △divIVA-3. The expression of the divIVA-2 gene was found to be higher as compared to the divIVA-1 and divIVA-3 genes in wild-type cells. The subcellular localisation studies revealed that the three different DivIVA proteins are spatially present at different regions of cell space. Our results suggest that an interplay of the three DivIVA proteins plays a role in cell shape maintenance in R. erythropolis PR4. Bioinformatics analysis and interactome study show that amongst the three copies of divIVA genes, the second copy, i.e., divIVA-2 gene, might have a more governing function in the cell division process as compared to the other two copies.
More Related Videos
12:29Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
Published on: March 11, 2022
09:24Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host
Published on: October 12, 2022
Related Concept Videos
Gene Conversion
Genome Copying Errors
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Molecular Factors Affecting Cell Division
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Mutations in Microorganisms
Other Unique Bacteria