Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
Experimental RNAi02:15

Experimental RNAi

6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
Homologous Recombination02:31

Homologous Recombination

52.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.0K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.5K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.5K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

24.6K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Staphylococcus aureus</i> ZigA is implicated in survival in zinc-deplete and genotoxic environments.

Microbiology spectrum·2026
Same author

FBH1 and RAD54L directly interact and cooperate to drive replication fork reversal.

bioRxiv : the preprint server for biology·2026
Same author

Multifaceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection.

Nature communications·2026
Same author

Structural insight into how RAD51 paralog exchange regulates RAD51 filament formation.

Nature structural & molecular biology·2026
Same author

Intrinsically disordered SERBP1 regulates translation through topology-driven G-quadruplex recognition.

bioRxiv : the preprint server for biology·2026
Same author

The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair.

Nucleic acids research·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Sep 12, 2025

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.1K

RAD52 and RPA act in a concert promoting inverse RNA strand exchange.

Sarah F DiDomenico, Hoang H Dinh, Matthew J Rossi

    Biorxiv : the Preprint Server for Biology
    |August 8, 2025
    PubMed
    Summary
    This summary is machine-generated.

    Replication protein A (RPA) significantly enhances RAD52

    More Related Videos

    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
    10:05

    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

    Published on: March 5, 2019

    6.6K
    Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
    07:24

    Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

    Published on: July 9, 2021

    2.5K

    Related Experiment Videos

    Last Updated: Sep 12, 2025

    Novel RNA-Binding Proteins Isolation by the RaPID Methodology
    11:19

    Novel RNA-Binding Proteins Isolation by the RaPID Methodology

    Published on: September 30, 2016

    9.1K
    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
    10:05

    Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

    Published on: March 5, 2019

    6.6K
    Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
    07:24

    Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

    Published on: July 9, 2021

    2.5K

    Area of Science:

    • Molecular Biology
    • DNA Repair Mechanisms
    • RNA-DNA Interactions

    Background:

    • Recent eukaryotic studies highlight RNA's role in DNA repair.
    • The RAD52 protein is identified as a key factor in RNA-dependent DNA repair.
    • Replication protein A (RPA) is known to stimulate RAD52's in vitro activities.

    Purpose of the Study:

    • To investigate the mechanism by which RPA stimulates RAD52's inverse RNA strand exchange activity.
    • To elucidate the specific interactions between RPA and RAD52 crucial for DNA repair.

    Main Methods:

    • Nuclear Magnetic Resonance (NMR) spectroscopy.
    • Biochemical assays to study protein interactions and enzymatic activity.
    • Characterization of RAD52 C-terminal domain (CTD) interactions.

    Main Results:

    • Identified two RPA-binding sites in the RAD52 C-terminal domain (CTD).
    • These sites mediate interactions with RPA70 and RPA32 subunits, critical for stimulation.
    • RPA-RNA complex formation is essential, strengthening RPA-RAD52 interaction and delivering RNA for strand exchange.

    Conclusions:

    • Elucidated the mechanism of RAD52-mediated inverse RNA strand exchange.
    • Demonstrated the critical role of the RPA-RAD52 interaction in RNA-dependent DNA repair.
    • Highlighted the function of RPA in facilitating RNA delivery for DNA repair processes.