Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.9K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.9K
Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Lineage Commitment01:21

Lineage Commitment

3.1K
Commitment is the  process whereby stem cells:
3.1K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CEACAM1 expression by immunohistochemistry in B-cell lymphomas and plasma cell myeloma.

American journal of clinical pathology·2026
Same author

Enhanced hypoxia resistance distinguishes human pluripotent stem cell-derived islets from primary islets.

American journal of physiology. Cell physiology·2026
Same author

Three-Year Follow-Up of Nivolumab-AVD Versus Brentuximab Vedotin-AVD in Adolescents With Advanced-Stage Classic Hodgkin Lymphoma on S1826.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Long-term culture of human pancreatic islets reveals reduced metal ion pathways in their gene signature.

Cell transplantation·2025
Same author

Nivolumab-AVD Versus Brentuximab Vedotin-AVD in Older Patients With Advanced-Stage Classic Hodgkin Lymphoma Enrolled on S1826.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2025
Same author

B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements.

Blood cancer discovery·2025

Related Experiment Video

Updated: Sep 12, 2025

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K

Methylation variability and LINE-1 activation in multiple myeloma.

Qianhui Wan1, Amy Leung1, Mahek Vinod Bhandari1

  • 1Department of Diabetes Complications and Metabolism, Beckman Research Institute.

Biorxiv : the Preprint Server for Biology
|August 8, 2025
PubMed
Summary

Multiple myeloma (MM) involves epigenetic changes, including DNA methylation loss, activating LINE-1 retrotransposons. This activation correlates with increased cell proliferation and decreased expression of silencing KRAB-zinc finger proteins (KZFPS).

More Related Videos

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.1K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.8K

Related Experiment Videos

Last Updated: Sep 12, 2025

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.1K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.8K

Area of Science:

  • Hematological Oncology
  • Epigenetics
  • Cancer Genomics

Background:

  • Multiple myeloma (MM) is a plasma cell cancer characterized by genetic mutations and epigenetic alterations.
  • Malignant plasma cells exhibit genome-wide DNA hypomethylation and increased active chromatin marks.
  • Epigenetic remodeling in cancer can lead to the reactivation of silenced transposable elements, impacting genome regulation.

Purpose of the Study:

  • To investigate the role of DNA methylation loss and transposable element activation in multiple myeloma.
  • To characterize the relationship between epigenetic changes, gene expression, and cellular states in MM.
  • To identify potential therapeutic targets related to epigenetic dysregulation in MM.

Main Methods:

  • Paired epigenome and transcriptome profiling of patient-derived MM samples.
  • Analysis of DNA methylation patterns, including partially methylated domains.
  • Quantification of LINE-1 (L1) retrotransposon activity and associated gene expression.
  • Assessment of KRAB-zinc finger protein (KZFP) abundance.

Main Results:

  • Loss of DNA methylation in MM creates patient-variable partially methylated domains.
  • This hypomethylation correlates with the expression of hundreds of LINE-1 (L1) retrotransposon-driven transcripts.
  • MM samples stratified by L1 activity show distinct gene expression signatures, with high L1 activity linked to proliferation and suppressed immune pathways.
  • Abnormally low abundance of KRAB-zinc finger proteins (KZFPS) was observed in MM samples with high L1 activity.

Conclusions:

  • Cell proliferation in multiple myeloma is associated with a loss of KZFP expression and subsequent activation of L1 elements.
  • L1 activation may contribute to the malignant phenotype in MM through various mechanisms, including oncogene transcription.
  • Epigenetic dysregulation, particularly L1 reactivation, represents a potential avenue for MM research and therapy.