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Updated: Sep 12, 2025
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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
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Comparative Proteomic Profiling of Receptor Kinase Signaling Reveals Key Trafficking Components Enforcing Plant
Pengfei Bai1,2, Minh Huy Vu1,2, Chiaki Komatsu1
1Howard Hughes Medical Institute, The University of Texas, Austin, TX 78712, USA.
Biorxiv : the Preprint Server for Biology
|August 8, 2025
Summary
Plant receptor kinases like ERECTA control development. This study reveals ERECTA interacts with endosomal trafficking machinery, essential for proper stomatal development and signaling.
Area of Science:
- Plant molecular biology
- Cell signaling
- Developmental biology
Background:
- Receptor kinases regulate crucial plant processes.
- ERECTA-family kinases are key in plant development, particularly stomatal formation.
- Understanding their regulation and dynamics is vital but limited.
Purpose of the Study:
- To elucidate the proteomic landscape of ERECTA-mediated signaling pathways.
- To compare the interactome and proximitome of ERECTA-family kinases.
- To identify novel regulators of ERECTA signaling and endocytosis.
Main Methods:
- Epitope-tagged affinity-purification mass spectrometry (ET-AP) to identify direct interactors.
- TurboID-based proximity labeling mass spectrometry (TbID-PL) to capture transient associations.
- Comparative proteomic analysis of ERECTA signaling network components.
Main Results:
- ET-AP identified known receptor complex members.
- TbID-PL revealed associations with endosomal trafficking, including clathrin-mediated endocytosis (CME) machinery.
- Specific phosphatidylinositol-binding clathrin assembly proteins (PICALMs) were identified as regulators of ERECTA internalization.
- PICALM mutations disrupted ERECTA endocytosis, causing excessive stomatal clustering.
Conclusions:
- A proteomic atlas of the ERECTA signaling network was generated.
- ERECTA kinase internalization via endocytosis is crucial for active signal transduction.
- This process is essential for correct stomatal patterning in plants.
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