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Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models
Published on: January 12, 2015
The misplaced mouse Pax6 interneuron subclass: A cross-species transcriptomic reassignment
Jonathan M Werner1, Hamsini Suresh1, Jesse Gillis1
1Physiology Department and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
Abstract:
Subclass cell-types are strongly conserved across mammalian brains, making the systemic absence of a caudal ganglionic eminence (CGE) Pax6 interneuron subclass in mouse transcriptional atlases unexpected. Through cross-species transcriptomic analysis, we identify a Pax6 subclass homolog in mouse and uncover primate-specific divergence in the Sncg subclass. Our results highlight the pitfalls of relying on single marker genes and provide evolution-aware annotations and marker sets to support robust cross-mammal interneuron comparisons.
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