Forensic biobank; towards comprehensive forensic genetic frequency database for the Kenyan population

Area of Science:

• Forensic Genetics
• Population Genetics
• Biobanking

Background:

• Forensic genetic frequency databases (FGFD) are crucial for estimating DNA match probabilities in legal cases.
• Existing databases recommended by the International Society for Forensic Genetics (ISFG) lack comprehensive data for certain populations, including Kenya.
• There is a need for updated, ethically sourced genetic data to support forensic investigations.

Purpose of the Study:

• To establish a forensic biobank for generating comprehensive genetic frequency data for the Kenyan population.
• To create a valuable resource for forensic genetics validation studies and population database enhancement.
• To ensure compliance with ethical standards in genetic data generation.

Main Methods:

• Collected 893 DNA samples from Kenyan volunteers, representing Bantu, Nilotic, and Cushitic populations.
• Stored samples as both FTA cards and extracted DNA for long-term preservation.
• Generated 132 quality mitogenome reference data for the coastal region.

Main Results:

• Developed a biobank with diverse Kenyan population representation (60.8% Bantu, 24.9% Nilotic, 14.3% Cushitic).
• Successfully generated initial mitogenome reference data for the coastal region.
• Established a resource for future generation of mitogenome, Y-chromosome, and autosomal STR data.

Conclusions:

• The established forensic biobank is a significant resource for generating essential genetic frequency data for Kenya.
• This resource will contribute to updating international forensic genetic databases and improving the probative value of forensic evidence.
• The biobank will support future forensic genetics research and validation studies, particularly with emerging technologies.