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Updated: Sep 12, 2025

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Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
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Tooth enamel as a forensic clock: A histological study evaluating enamel structures for forensic age assessment using
Divya Shetty1, Shanvi Kumari2, Nikita Gulati2
1Department of Orthodontics and Dentofacial Orthopaedics and Research, I.T.S. Centre for Dental Studies and Research, Muradnagar, Ghaziabad, U.P, 201206, India.
Journal of Oral Biology and Craniofacial Research
|August 8, 2025
Summary
Tooth enamel
Area of Science:
- Forensic odontology
- Dental histology
- Age estimation
Background:
- Dental evidence is reliable in forensic investigations.
- Tooth enamel's durability aids postmortem analysis.
- Histological features of enamel may indicate age, species, or gender.
Purpose of the Study:
- To evaluate histological enamel structures for age assessment.
- To determine the accuracy of enamel features in predicting age.
Main Methods:
- Analysis of 120 premolar samples (ages 12-55).
- Stereomicroscopic examination of linear enamel hypoplasia.
- Ground sectioning for lamellae counting and polarizing microscopy for hypo-mineralization zones.
Main Results:
- The C5.0 predictive model achieved the highest accuracy (85.30%) for age determination.
- Lamellae number was the most significant predictor of age.
- Predictive accuracy varied across age groups, with higher accuracy for younger groups.
Conclusions:
- Unique enamel profiles can assist in identifying individuals from mass disasters or damaged remains.
- Routine documentation of enamel defects by dentists is recommended for forensic comparisons.
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