Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Corrosion01:24

Microbial Corrosion

67
Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
67
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

132
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
132
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

54
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
54

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating Variability in Extracellular Vesicle Characterization Across Measurement Techniques.

Journal of extracellular biology·2026
Same author

Dynamics of particles suspended in field-enhanced microscale flows.

Soft matter·2026
Same author

In silico and in vitro insights into stigmasterol targeting Keap1/Nrf2, Bcl-2/Bax and IKKβ/IκBα protein-protein interactions in Arsenic-induced toxicity.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

The evolution of next-generation lateral flow assays for bacterial and fungal diagnostics.

Mikrochimica acta·2026
Same author

In-Silico and Functional Characterization of EcdLp, an ABC Transporter of Aspergillus nidulans NRRL11440.

Current microbiology·2026
Same author

Functional Abnormalities of the Endocrine System in Beta-Thalassemia Major Patients: Insights From a Hospital-Based Observational Study.

Cureus·2026

Related Experiment Video

Updated: Apr 12, 2026

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
07:40

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles

Published on: June 2, 2017

15.2K

Silver Nanoparticles Synthesis From Bacillus subtilis and Its ROS-Mediated Staphylocidal Activity Against

Minakshi Sinha1, Hemlata Kumari1, Shaurya Prakash1

  • 1Department of Biochemistry, Central University of Haryana, Mahendergarh, India.

Journal of Basic Microbiology
|August 8, 2025
PubMed
Summary

Green synthesized silver nanoparticles (AgNPs) show potent antimicrobial activity against drug-resistant bacteria like MRSA. These AgNPs reduce bacterial susceptibility and inhibit biofilm formation, offering a promising avenue for combating antimicrobial resistance (AMR).

Keywords:
ROSStaphylococcus aureusantimicrobial‐resistantbiofilmcharacterizationsilver nanoparticles

More Related Videos

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
11:19

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Published on: May 10, 2018

10.3K
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.3K

Related Experiment Videos

Last Updated: Apr 12, 2026

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
07:40

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles

Published on: June 2, 2017

15.2K
Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
11:19

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Published on: May 10, 2018

10.3K
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.3K

Area of Science:

  • Nanotechnology
  • Microbiology
  • Materials Science

Background:

  • Antimicrobial resistance (AMR) in Staphylococcus aureus, including MRSA and VRSA, poses a significant global health threat, complicating treatment in clinical settings.
  • The rise in drug-resistant infections, particularly in middle-income countries, is exacerbated by high hospitalization rates and improper antibiotic use, leading to increased morbidity and mortality.
  • Nanotechnology offers innovative solutions for combating acute and chronic microbial infections, with silver nanoparticles (AgNPs) showing particular promise.

Purpose of the Study:

  • To synthesize silver nanoparticles (AgNPs) using a green chemistry approach with Bacillus subtilis cell-free extract.
  • To characterize the synthesized AgNPs using various spectroscopic and imaging techniques.
  • To evaluate the antimicrobial efficacy of AgNPs against multidrug-resistant pathogens, including methicillin-resistant Staphylococcus aureus (MRSA).

Main Methods:

  • Green synthesis of AgNPs using Bacillus subtilis cell-free extract.
  • Characterization of AgNPs via UV-VIS, FT-IR, SEM, TEM, zeta potential, XRD, and EDX analysis.
  • Antimicrobial efficacy testing against Gram-negative and Gram-positive bacteria, including MRSA, determining minimum inhibitory concentration (MIC), and assessing bactericidal effects through ROS production, macromolecule leakage, and biofilm inhibition.

Main Results:

  • Characterization confirmed the successful synthesis of AgNPs.
  • AgNPs demonstrated significant antimicrobial potency against MRSA with a minimum inhibitory concentration (MIC) of 0.4 mg/mL and were effective against other tested pathogens.
  • AgNPs exhibited bactericidal effects by inducing reactive oxygen species (ROS) production and macromolecule leakage, and effectively inhibited biofilm formation by up to 82% at 1.6 mg/mL concentration.

Conclusions:

  • Green synthesized AgNPs possess potent antimicrobial activity against a broad spectrum of bacterial pathogens, including drug-resistant strains like MRSA.
  • The antimicrobial mechanism involves increasing cellular susceptibility through ROS generation and promoting DNA and protein leakage.
  • AgNPs effectively inhibit bacterial biofilm formation, highlighting their potential to combat the growing challenge of antimicrobial resistance in Staphylococcus aureus infections.