A Transparent, Antimicrobial, Bio-Ionic Liquid Functionalized Hydrogel for Corneal Injury Repair

Area of Science:

• Biomaterials Science
• Ophthalmology
• Regenerative Medicine

Background:

• Current corneal repair methods like suturing and cyanoacrylate glue have significant adverse effects, hindering healing.
• These complications include astigmatism, necrosis, infection, and fluid leakage, necessitating improved therapeutic strategies.

Purpose of the Study:

• To develop and evaluate an innovative bioadhesive hydrogel, BioPEG, for enhanced corneal repair and regeneration.
• BioPEG aims to overcome the limitations of existing treatments by providing transparency, adhesion, flexibility, and antimicrobial properties.

Main Methods:

• BioPEG hydrogel was synthesized using visible light photoconjugation of polyethylene glycol diacrylate (PEGDA) and a cholinium-based bio-ionic liquid (BIL).
• The hydrogel's performance was assessed ex vivo and in vivo using rabbit and porcine corneal stromal defect models.
• Infection suppression and re-epithelialization capabilities were evaluated in models infected with Staphylococcus aureus and Pseudomonas aeruginosa.

Main Results:

• BioPEG demonstrated high corneal adhesion, strength, and promoted significant repair and regeneration in corneal defect models.
• The hydrogel effectively suppressed bacterial infection caused by Staphylococcus aureus and Pseudomonas aeruginosa.
• BioPEG promoted re-epithelialization, indicating its safety and efficacy in promoting corneal healing.

Conclusions:

• BioPEG represents a safe and effective alternative for rapid corneal repair and regeneration.
• Its unique properties make it superior to current standard treatments for corneal injuries.
• This bioadhesive hydrogel platform holds significant promise for clinical applications in ophthalmology.