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Interpretive standards and quality control guidelines for cefpiramide disk susceptibility tests.

A L Barry, T L Gavan, C Thornsberry

    Journal of Clinical Microbiology
    |October 1, 1985
    PubMed
    Summary
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    This study recommends using 75-micrograms cefpiramide disks for bacterial susceptibility testing. Established zone size standards help determine bacterial resistance to this antibiotic.

    Area of Science:

    • Microbiology
    • Clinical Pharmacy
    • Antibiotic Resistance

    Background:

    • Accurate antibiotic susceptibility testing (AST) is crucial for guiding effective treatment strategies.
    • Cefpiramide is a cephalosporin antibiotic used to treat various bacterial infections.
    • Standardized disk diffusion methods are widely employed for AST, but require validation for new antimicrobial agents or disk concentrations.

    Purpose of the Study:

    • To evaluate the performance of 30- and 75-micrograms cefpiramide disks in disk susceptibility tests.
    • To establish quality control parameters and recommend appropriate zone size interpretive criteria for cefpiramide disk diffusion testing.
    • To provide evidence-based recommendations for the clinical laboratory use of cefpiramide disks.

    Main Methods:

    Related Experiment Videos

  • Disk susceptibility tests were performed on 614 bacterial isolates using cefpiramide disks of 30- and 75-micrograms concentrations.
  • Bacterial isolates were tested against cefpiramide to determine their susceptibility profiles.
  • Minimum Inhibitory Concentration (MIC) values were correlated with zone diameters obtained from disk diffusion tests.

    • Main Results:

    • The study evaluated the efficacy of two different cefpiramide disk concentrations against a large panel of bacterial isolates.
    • Recommended zone size standards were proposed: ≥19 mm for susceptible (MIC ≤32 µg/ml) and ≤15 mm for resistant (MIC ≥128 µg/ml) using 75-micrograms disks.
    • Quality control parameters for these disk tests were assessed and control limits were suggested.

    Conclusions:

    • The 75-micrograms cefpiramide disk test is a reliable method for determining bacterial susceptibility.
    • The proposed zone size standards provide clear interpretive criteria for cefpiramide's clinical utility.
    • Implementation of these validated disk diffusion methods can aid in optimizing antibiotic therapy and combating antimicrobial resistance.