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Updated: Sep 12, 2025

In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
Combined whole-genome resequencing with transcriptomic profiling reveals the genetic basis of plant architecture in
Xin Wang1, Mengjiao Shen1, Zhiting Wan1
1State Key Laboratory of Efficient Production of Forest Resources, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China.
Abstract:
Plant height and branching are critical architectural traits in woody plants, influencing plant appearance and ornamental value. To elucidate the regulatory mechanisms, we investigated the genetic basis of plant architecture in Lagerstroemia indica by comparing a low-compact cultivar 'Fenjingling' with its tall mutants. Phenotypic and cytological traits were measured, single nucleotide polymorphisms (SNPs), insertions/deletions (InDels) and structural variations (SVs) were identified by whole-genome resequencing (WGRS), and the key variants were validated by Sanger sequencing and transcriptomic data. The results showed that compared with 'Fenjingling', the plant height, xylem cell number, xylem cell length, pith cell number, and pith cell length of the mutant increased by 3.4, 4.2, 1.9, 3.4, and 1.6 times, respectively; while the number of branches and internodes decreased by 68.49 % and 47.36 %, respectively. The coding sequences (CDS) variations of WRKY40 between 'Fenjingling' and its mutants were related to plant height. Virus-induced gene silencing (VIGS) results confirmed that a 343 bp region sequence in WRKY40 caused functional alteration, and regulated plant height by regulating cell proliferation and growth. The results provided new insights into the genetic mechanisms of plant architecture in crape myrtle.
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