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Updated: Sep 12, 2025
Viral Nanoparticles for In vivo Tumor Imaging
Published on: November 16, 2012
Storage stability of cowpea mosaic virus cancer drug candidate - a two-year update
Andrea Simms1,2,3,4, Narek Minasov1,2,3,4, Nicole F Steinmetz1,2,3,4,5,6,7,8
1Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, CA, USA.
Aim:
To evaluate the structural stability and biological activity of cowpea mosaic virus (CPMV) after 2 years of storage.
Materials And Methods:
CPMV was stored at room temperature (RT), 4°C, and -20°C for 2 years. Structural stability was assayed using dynamic light scattering (DLS), size exclusion chromatography (SEC), native and denaturing gel electrophoresis, and transmission electron microscopy (TEM). Biological activity was assessed by anti-tumor efficacy studies using a B16F10 dermal mouse melanoma model and by a cowpea plant infection challenge.
Results:
Storage at -20°C preserved CPMV's structural properties and biological activity. Storage at 4°C caused aggregation, S-protein cleavage, and RNA degradation - samples stored at 4°C had decreased anti-tumor efficacy yet retained plant infectivity. CPMV stored at RT was degraded and lost biological activity.
Conclusion:
Frozen storage allows CPMV to maintain structural and biological stability for at least 2 years.
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