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Updated: Sep 12, 2025
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In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
Published on: July 11, 2019
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A basophil-fibroblast pro-inflammatory axis fuels type 2 skin inflammation.
Ichiro Imanishi1, Raman Gill2, Alexis Wilder1
1Kimberly and Eric J. Waldman Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Cell Reports
|August 9, 2025
Summary
Researchers mapped skin inflammation at single-cell resolution, revealing a key communication pathway between basophils and fibroblasts. This discovery offers new therapeutic targets for chronic inflammatory skin diseases like atopic dermatitis.
Area of Science:
- Immunology
- Dermatology
- Systems Biology
Background:
- Chronic inflammatory skin diseases involve complex cell interactions hindering effective treatment.
- Understanding these cellular dynamics is crucial for developing targeted therapies.
Purpose of the Study:
- To create a comprehensive single-cell spatiotemporal atlas of murine type 2 skin inflammation.
- To identify key cellular players and their interactions in inflammatory skin conditions.
Main Methods:
- Utilized MERFISH and single-cell RNA sequencing (scRNA-seq) to analyze approximately 430,000 cells.
- Induced dermatitis in mice using MC903 and oxazolone to model skin inflammation.
- Performed spatial neighborhood analyses to understand cell-cell communication.
Main Results:
- Identified 39 distinct cell types, including pro-inflammatory fibroblasts similar to those in human atopic dermatitis.
- Discovered basophils activate pro-inflammatory fibroblasts, with basophil-derived oncostatin-M (OSM) and IL-4 driving a positive feedback loop.
- Demonstrated that targeting the IL-4Rα receptor on fibroblasts or inhibiting gp130 (OSM receptor component) significantly reduces inflammation.
Conclusions:
- Established a critical basophil-fibroblast communication circuit regulating type 2 skin inflammation.
- Redefined the role of basophils in inflammation and highlighted fibroblasts as dynamic immune regulators.
- Identified this circuitry as a potential therapeutic target for inflammatory skin diseases.
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