Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

40
Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
40
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care01:30

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

36
A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
36
Endocarditis III: Medical Management01:18

Endocarditis III: Medical Management

17
Infective endocarditis management involves a multifaceted approach encompassing infection prevention, lifestyle modifications, pharmacological therapy, and surgical management.Infection Prevention:Hand Hygiene: Thorough handwashing is crucial to prevent the spread of infection. Hand hygiene should be performed regularly, especially before and after using the restroom.Oral Hygiene: Good oral hygiene is essential. It includes brushing teeth immediately after waking up and before bed, flossing...
17
Endocarditis I: Introduction01:25

Endocarditis I: Introduction

26
Introduction:Endocarditis is the infection of the endocardium, the inner lining of the heart and its valves. When the heart muscle is involved, the condition is termed myocarditis, while an infection of the outer lining is called pericarditis. Infective endocarditis (IE) primarily affects the endocardium, where pathogens adhere to the valves or lining, forming vegetation that can lead to severe complications. Infective endocarditis occurs when microorganisms, usually bacteria from other body...
26
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.2K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.2K
Bacterial Signaling01:30

Bacterial Signaling

34.2K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
34.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pre-operative Patient Optimization Reduces Complications Following Total Knee Arthroplasty: An Evidenced-based Approach.

The Orthopedic clinics of North America·2025
Same author

Novel Technologies in Periprosthetic Joint Infections: Emerging Therapeutics.

The Journal of arthroplasty·2025
Same author

Patient Perception of Preoperative Nutrition and Recovery After Orthopaedic Surgery.

The Journal of arthroplasty·2024
Same author

Achieving Correct Balance in Total Knee Arthroplasty with Fixed Varus Deformity.

The journal of knee surgery·2023
Same author

Surgical Trends for Managing Knee Osteonecrosis: A 2010 to 2020 United States Nationwide Study.

The Journal of arthroplasty·2023
Same author

A nationwide comparative analysis of medical complications in fibromyalgia patients following total knee arthroplasty.

Annals of translational medicine·2019

Related Experiment Video

Updated: Sep 12, 2025

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.2K

Novel Technologies in Periprosthetic Joint Infection: Emerging Diagnostics.

Matthew L Magruder1, Nathanael D Heckmann2, Jay R Lieberman2

  • 1Department of Orthopaedic Surgery, Maimonides Medical Center, Brooklyn, New York.

The Journal of Arthroplasty
|August 9, 2025
PubMed
Summary

Novel diagnostic tools like pH sensors and calprotectin assays offer faster, more accurate detection of periprosthetic joint infection (PJI) after total joint arthroplasty (TJA). These methods improve patient outcomes by enabling earlier PJI diagnosis and treatment.

Keywords:
MSIS criteriabiofilmdiagnosisprosthetic joint infectiontechnology

More Related Videos

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.5K
Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections
04:37

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Published on: February 2, 2024

926

Related Experiment Videos

Last Updated: Sep 12, 2025

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.2K
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

1.5K
Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections
04:37

Author Spotlight: Advancing Research on Candida albicans Biofilm-Associated Prosthetic Joint Infections

Published on: February 2, 2024

926

Area of Science:

  • Orthopedic Surgery
  • Infectious Diseases
  • Biomarker Discovery
  • Diagnostic Technologies

Background:

  • Periprosthetic joint infection (PJI) is a significant complication of total joint arthroplasty (TJA), leading to high morbidity and implant failure.
  • Current diagnostic methods for PJI, such as synovial fluid analysis and cultures, have limitations in sensitivity and specificity, especially for culture-negative cases.
  • There is a critical need for advanced diagnostic technologies for rapid and reliable PJI detection.

Purpose of the Study:

  • To review and explore emerging diagnostic strategies for periprosthetic joint infection (PJI).
  • To highlight novel technologies that offer improved accuracy and speed in PJI diagnosis.
  • To discuss the potential impact of these innovations on patient outcomes and prosthetic longevity.

Main Methods:

  • Review of emerging diagnostic strategies including real-time intra-articular pH monitoring (e.g., X-ray-based sensors, XELCI).
  • Evaluation of urinary peptide biomarkers for systemic inflammatory response detection.
  • Assessment of multiomics techniques (transcriptomics, proteomics, immune cell profiling) and advanced synovial fluid assays, focusing on calprotectin.

Main Results:

  • Implantable pH sensors and XELCI enable noninvasive, early detection of synovial environment changes indicative of infection.
  • Urinary peptide biomarkers offer a noninvasive method to reflect systemic inflammation associated with PJI.
  • Synovial calprotectin, detected via lateral flow assay, shows high sensitivity and specificity for differentiating PJI from aseptic failures, serving as a point-of-care tool.

Conclusions:

  • Emerging diagnostic technologies, including pH monitoring, urinary biomarkers, multiomics, and synovial calprotectin assays, show promise for revolutionizing PJI detection.
  • These novel methods have the potential to reduce diagnostic delays and the need for invasive procedures.
  • Further large-scale clinical validation is necessary to establish these innovations as standard-of-care for improved patient outcomes and prosthetic joint survival.