Development and selection of stably expressed reference genes for expression normalization in Ribes odoratum under...

Area of Science:

• Molecular Biology
• Plant Science
• Genetics

Background:

• Accurate gene expression analysis using quantitative PCR (qPCR) relies on stable internal reference genes for normalization.
• Limited validation studies for reference genes in Ribes odoratum hinder reliable gene function analysis, especially under environmental stresses like drought.

Purpose of the Study:

• To identify and validate stable reference genes for normalizing qPCR data in Ribes odoratum under drought stress conditions.
• To establish a reliable foundation for future molecular biology research in Ribes odoratum.

Main Methods:

• Drought stress treatments were applied to Ribes odoratum.
• Transcriptome analysis was performed to identify candidate reference genes.
• Expression stability of 17 candidate reference genes was evaluated using multiple algorithms (Genorm, NormFinder, BestKeeper, Delta Ct).

Main Results:

• Multiple genes with stable expression under drought stress were identified.
• Cluster-37.22228, Cluster-37.22646, and Cluster-37.22897 demonstrated the highest expression stability.
• Cluster-37.22228 and Cluster-37.22646 were identified as the optimal reference genes for Ribes odoratum under drought stress.

Conclusions:

• This study provides the first comprehensive list of validated reference genes for qPCR in Ribes odoratum under drought stress.
• The identified stable reference genes will enhance the accuracy and reliability of gene expression studies in this species.
• These findings will facilitate future molecular research and genetic studies in Ribes odoratum.