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Updated: Sep 12, 2025
10:27
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Published on: December 5, 2019
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Rice RING E3 Ligase OsRMT1 Negatively Regulates Salt Tolerance by Degrading the Canonical Salt-Responsive Protein
Wenjuan Zhang1, Kun Jing1, Jun Zhu1
1Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, Yangzhou, Jiangsu, China.
Plant Biotechnology Journal
|August 10, 2025
Summary
Rice plants utilize OsMBL1 for salt tolerance. A protein, OsRMT1, degrades OsMBL1, negatively impacting salt response. Understanding this OsRMT1-OsMBL1 interaction is key for improving crop resilience to salinity.
Area of Science:
- Plant Biology
- Molecular Biology
- Agricultural Science
Background:
- Excessive salinity is a major abiotic stressor limiting plant growth and agricultural yields.
- The function and regulation of rice jacalin-related mannose-binding lectin (OsMBL1) under salt stress are not fully understood.
Purpose of the Study:
- To elucidate the role of OsMBL1 in rice salt tolerance.
- To identify regulatory mechanisms governing OsMBL1 activity in response to salinity stress.
Main Methods:
- Gene knockout and overexpression in rice (Oryza sativa).
- Heterologous expression in *Solanum lycopersicum*, *Brassica napus*, and *Arabidopsis*.
- Yeast two-hybrid (Y2H) screening, co-immunoprecipitation (Co-IP) assays (in vivo and in vitro).
- Ubiquitination assays and genetic analyses.
Main Results:
- OsMBL1 acts as a positive regulator of salt tolerance in rice.
- OsMBL1 overexpression enhances salt tolerance, while knockout lines show reduced tolerance and increased H2O2 levels.
- OsRMT1, a RING E3 ubiquitin ligase, interacts with OsMBL1 and negatively regulates salt tolerance by promoting OsMBL1 degradation via ubiquitination.
- OsRMT1 functions upstream of OsMBL1, influencing downstream salt-tolerance gene expression.
Conclusions:
- The OsRMT1-OsMBL1 regulatory module is crucial for the rice response to salt stress.
- OsRMT1-mediated degradation of OsMBL1 is a key mechanism controlling salt tolerance.
- This study provides insights into molecular mechanisms for enhancing plant salt tolerance.
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