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Transcriptome to reveal the immunological defenses in mandarin fish (Siniperca chuatsi) skin with LPS and poly (I:C)
Yawei Shen1, Jiong Gao2, Meng Zhang3
1College of Fisheries, Henan Normal University, Xinxiang, 453007, Henan, China; Department of Biological Sciences, Clemson University, Clemson, SC, 29634, USA; Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province, Nanyang, 474450, China.
Abstract:
The skin mucosa of fish serves as a vital immune barrier, offering both anatomical and physiological protection against external pathogens. This study explored gene expression profiles and potential immune signaling pathways in the skin of mandarin fish (Siniperca chuatsi) following stimulation with LPS or poly (I:C) at 0 h, 3 h, 6 h, and 12 h. Results revealed 648 shared differentially expressed genes (DEGs) across LPS treatment groups and 484 shared DEGs under poly (I:C) treatment. Among these, 288 DEGs were consistently regulated across all conditions. Functional analysis identified the MAPK signaling pathway and cytokine-cytokine receptor interaction as the most significantly enriched and suppressed pathways. The DEGs within the MAPK signaling pathway (dusp1, dusp2, dusp5, dusp8a, jun, jund-a, jund-b, jun-l, fosab, epha2a, nr4a1, nr4a1l, hras, il1b, fgf1, nfatc1, and prkacab) and cytokine-cytokine receptor interaction (tnfrsf9, tnfrsf10b, tnfrsf12a, il10ra, il21r, il1b, pf2) were significantly changes in all compared groups. The protein-protein interactions analysis suggested that jun was the hub gene. The candidate's genes and pathways identified in this study may, following validation, promote the mandarin fish skin immune response study offer insights into fish mucosal immunity and may support future vaccine development strategies.
