Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.1K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.1K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

688
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
688

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D Bioprinting of High-Grade Serous Ovarian Cancer Cells: Workflow for Gelatin-Based Bioink Formulation with Hyaluronic Acid and Printability Assessment.

ACS applied bio materials·2026
Same author

Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.

ACS applied nano materials·2026
Same author

Towards greener-by-design fine chemicals. Part 2: technological frontiers.

Chemical Society reviews·2025
Same author

pH-Thermo Dual-Responsive Polymeric Nanoparticles for Women's Health: Dual Action Against Cervical and Ovarian Cancer Cells.

ACS applied materials & interfaces·2025
Same author

Drug Delivery from Nanogel Formulations: Mathematical Modeling of Different Release Profiles and Conditions.

Molecular pharmaceutics·2025
Same author

Fluorinated Redox-Responsive Cross-Linked Poly(amidoamine) G2 as Smart Theranostic Dendrimers.

Biomacromolecules·2025

Related Experiment Video

Updated: Sep 11, 2025

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

11.4K

3D Printed Microfluidic Chromatographic Column for Fast Downstream Processing Development.

Vladimir Matining1, Mario Messina1, Benedetta Sechi1

  • 1Department of Chemistry, Materials, and Chemical Engineering "Giulio Natta", Politecnico Di Milano, Piazza Leonardo Da Vinci 32, Milan, Italy.

Biotechnology Journal
|August 11, 2025
PubMed
Summary

3D printing enables novel microfluidic chromatographic columns for efficient biomolecule analysis. This study developed a 3D printed column for lysozyme characterization, reducing sample and resin requirements.

Keywords:
3D printingHETPadsorption equilibriumchromatographyhigh‐throughput screeningmicrofluidics

More Related Videos

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.1K
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K

Related Experiment Videos

Last Updated: Sep 11, 2025

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

11.4K
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.1K
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K

Area of Science:

  • Analytical Chemistry
  • Chemical Engineering
  • Biotechnology

Background:

  • Microfluidic devices offer advantages in reduced sample consumption and faster analysis.
  • 3D printing provides a versatile platform for fabricating complex microfluidic structures.

Purpose of the Study:

  • To develop and characterize a 3D printed microfluidic chromatographic column.
  • To evaluate the column's performance for protein characterization, specifically lysozyme.
  • To establish a mechanistic model for protein transport in the microcolumn.

Main Methods:

  • Fabrication of a 54 µL microfluidic column using 3D printing.
  • Packing the column with cation exchange resin.
  • Characterization using potassium iodide tracer and lysozyme.
  • Analysis of adsorption isotherms and axial dispersion.

Main Results:

  • The 3D printed column exhibited good chromatographic properties (porosity ε=0.72, asymmetry factor 0.8-1.8).
  • Lysozyme saturation capacity was measured at 88.14 g/L resin.
  • A transition from Langmuir to anti-Langmuir adsorption was observed at high NaCl concentrations (≥300 mM).
  • Axial dispersion coefficient was determined as 6.7 x 10⁻⁹ m²/s.

Conclusions:

  • 3D printing is a viable technique for creating high-performance microfluidic chromatography columns.
  • The developed microcolumn enables comprehensive characterization of biomolecules like lysozyme with minimal sample and resin.
  • This approach streamlines process development and addresses the challenge of limited biomolecule availability.