Prolonged chimeric antigen receptor-T apheresis to infusion time is associated with inferior outcomes in diffuse...

Chimeric antigen receptor (CAR) T-cell therapy is effective for relapsed/refractory (r/r) diffuse large B-cell lymphoma (DLBCL). However, delays between apheresis and infusion frequently occur due to limited facility capacity and prolonged bridging therapy, and the clinical impact of such delays remains uncertain. We retrospectively analysed R/R DLBCL patients who underwent CAR-T-cell therapy at Kyoto University Hospital. Among 90 patients, the median apheresis to infusion interval was 66 days (range, 28-203). Patients were categorized into delayed (≥66 days) and non-delayed (<66 days) groups. Baseline characteristics at infusion were similar, whereas adverse features such as extranodal involvement and stable/progressive disease (SD/PD) were less common in the delayed group. Multivariate analysis identified delayed infusion as a significant negative prognostic factor for progression-free survival (adjusted hazard ratio [aHR] 3.13; 95% confidence interval [CI] 1.63-6.00; p = 0.001), along with extranodal involvement (aHR 2.39; p = 0.004), SD/PD (aHR 2.66; p = 0.005), bulky disease (aHR 3.08; p = 0.008) and treatment with tisa-cel (aHR 5.26; p = 0.001). Overall survival was also inferior in the delayed group (aHR 2.53, 95% CI 1.29-4.96; p = 0.007). Minimizing apheresis to infusion interval through improved workflows, institutional capacity and avoiding prolonged bridging in non-responders may enhance outcomes.