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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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  1. Home
  3. Integrated Analysis Of Single-cell And Bulk Rna Sequencing Data Reveals Cellular Senescence-related Signatures Predicting New Potential Therapeutic Drugs In Hepatic Ischemia-reperfusion Injury.
  1. Home
  3. Integrated Analysis Of Single-cell And Bulk Rna Sequencing Data Reveals Cellular Senescence-related Signatures Predicting New Potential Therapeutic Drugs In Hepatic Ischemia-reperfusion Injury.

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Integrated Analysis of Single-Cell and Bulk RNA Sequencing Data Reveals Cellular Senescence-Related Signatures

Junlin Chen1, Huaming Huang2, Zhi Lu3,4

  • 1Department of Gastroenterology and Hepatology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

IUBMB Life
|August 11, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
cellular senescencefluoxetinehepatic ischemia reperfusion injuryimmune cellssingle‐cell RNA sequencing

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This study identified key genes involved in cellular senescence during hepatic ischemia-reperfusion injury (IRI). Fluoxetine (FLX) was found to mitigate liver injury by suppressing these senescence-related factors, offering a potential therapeutic strategy for IRI.

Area of Science:

  • Hepatology
  • Immunology
  • Genomics

Background:

  • Hepatic ischemia-reperfusion injury (IRI) is a significant clinical challenge.
  • Cellular senescence plays a role in IRI pathogenesis, but the underlying molecular mechanisms are not fully understood.

Purpose of the Study:

  • To identify differentially expressed genes related to cellular senescence in hepatic IRI.
  • To explore the role of immune cells and their interactions in IRI.
  • To identify potential therapeutic agents for hepatic IRI.

Main Methods:

  • Analysis of human bulk RNA sequencing (RNA-seq) data from IRI patients and controls.
  • Single-cell RNA sequencing (scRNA-seq) analysis of a mouse hepatic IRI model.
  • Bioinformatic predictions including drug-gene interactions, molecular docking, and simulations.
  • Experimental validation using in vivo mouse models and in vitro cell assays.

    • Main Results:

    • 19 differentially expressed genes related to cellular senescence (DEG-CSRGs) were identified, with hub genes like JUN, FOS, and ATF3 highlighted.
    • Macrophages, monocytes, and neutrophils were identified as key immune cells in hepatic IRI, showing upregulated hub gene expression.
    • Fluoxetine (FLX) was predicted as a potential therapeutic agent and experimentally validated to mitigate liver injury and suppress senescence-related factors.

    Conclusions:

    • Cellular senescence and specific immune cell populations are critical in hepatic IRI.
    • Fluoxetine demonstrates therapeutic potential for mitigating liver injury in IRI by targeting senescence pathways.