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Updated: Sep 11, 2025
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
Effect of Sheng Xuexiaoban capsules on Tregs in an ITP mouse model
Jianqiang Liu1, Xiaoyan Li2, Shenggui Hou3
1Department of Joint Surgery, The Fourth People's Hospital, Jinan, China.
Objectives:
To study the effect of Sheng Xuexiaoban capsules (SXXCs) on restoring immune dysfunction in ITP model mice.
Methods:
We assessed changes in platelet counts, megakaryocyte counts, Treg functions, interleukin-2 expression, and other indicators to investigate the effect of SXXCs in the ITP mouse model.
Results:
After treatment of the ITP mouse model with SXXCs, we found increases in platelets (577.35 ± 76.94 vs. 123.33 ± 16.24, P < 0.05) and the Treg number (7.35 ± 1.94 vs. 1.33 ± 1.24), and Foxp3 mRNA expression (2-ΔCt: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, P = 0.0027). Decreased interleukin-2 produced by effector T cells was detected (2-ΔCt: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, P < 0.001). SXXCs treatment significantly improved the proportion and function of Tregs. By increasing Foxp3 mRNA expression and decreasing interleukin-2, we restored immune tolerance to achieve a good therapeutic effect in ITP model mice.
Conclusions:
We confirmed that SXXCs have a good therapeutic effect on ITP. Simultaneously we verified the important role of Tregs in ITP pathogenesis.
