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Microfluidic Platform for Predicting Nanoparticle Dynamics in Cancer Therapeutics.

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This study developed a cost-effective microfluidic tumor-on-a-chip for nanoparticle (NP) cancer treatment research. It investigated synthetic and green synthesized silver nanoparticles (AgNPs) for breast cancer cell viability, showing promising results for chemotherapy applications.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Research

Background:

  • Microfluidic devices offer a controlled environment for studying cellular interactions.
  • Nanoparticles (NPs) show potential in targeted cancer therapy, but their behavior in biological systems needs thorough investigation.
  • Developing cost-effective and adaptable platforms is crucial for advancing cancer treatment research.

Purpose of the Study:

  • To design a simple, cost-effective microfluidic tumor-on-a-chip model.
  • To investigate the penetration and behavior of synthetic and green synthesized nanoparticles (NPs) for cancer treatment.
  • To evaluate the efficacy of silver nanoparticles (AgNPs) on breast cancer cell viability.

Main Methods:

  • Characterization of synthetic Yellow-Green and Red FluoSphere NPs and green synthesized AgNPs using Dynamic Light Scattering (DLS), zeta potential analysis, UV-Vis spectrophotometry, FTIR, XRD, and Transmission Electron Microscopy (TEM).
  • Design and utilization of a microfluidic tumor-on-a-chip device.
  • Assessment of NP penetration using Fluorescence Microscopy and cell viability using MTT assay and Live/dead kit on MCF7 breast cancer cells.

Main Results:

  • Red FluoSpheres exhibited lower penetration intensity (42%) compared to Yellow-Green FluoSpheres (49%).
  • Off-chip MTT assay showed highest cell viability at 35% with a 2:1 ratio of AgNPs.
  • On-chip experiments indicated cell viability at 30% with 100 µM AgNPs, verified by Live/dead kit.

Conclusions:

  • The developed microfluidic tumor-on-a-chip is a convenient and cost-effective platform for NP-based cancer research.
  • Both synthetic and green synthesized NPs demonstrate potential for cancer treatment applications.
  • Further investigation into various NP formulations is warranted for optimizing chemotherapy and related applications.