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Related Experiment Video

Updated: Sep 11, 2025

Optimized Minimally Invasive Transscleral Subretinal Injection Technique in Mouse
06:48

Optimized Minimally Invasive Transscleral Subretinal Injection Technique in Mouse

Published on: July 25, 2025

228

Optimized Minimally Invasive Transscleral Subretinal Injection Technique in Mouse.

Lauren Y Cao1, Lindsey A Chew2, Stella Finkelstein1

  • 1Department of Ophthalmology, Duke University School of Medicine.

Journal of Visualized Experiments : Jove
|August 11, 2025
PubMed
Summary
This summary is machine-generated.

We optimized a minimally invasive transscleral injection technique for subretinal delivery in mice. This method preserves retinal function and minimizes damage, improving therapeutic efficacy assessments for retinal diseases.

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Area of Science:

  • Ophthalmology
  • Retinal Surgery
  • Gene Therapy Delivery

Background:

  • Conventional subretinal injections in mice cause significant retinal damage.
  • This damage introduces variability in functional outcome measures, complicating therapeutic assessments.
  • Existing methods hinder the development of treatments for retinal and macular diseases.

Purpose of the Study:

  • To optimize a minimally invasive transscleral subretinal injection technique in mice.
  • To compare the functional and anatomical outcomes of this new technique against the conventional transretinal method.
  • To establish a more reliable method for delivering therapeutics to the subretinal space.

Main Methods:

  • Developed a transscleral injection technique accessing the superior fornix.
  • Utilized a custom eyelid speculum, diamond knife for sclerotomy, and microinjection pump.
  • Assessed subretinal bleb with optical coherence tomography (OCT) and retinal function via electroretinogram (ERG).

Main Results:

  • Transscleral injection preserved retinal function, with ERG results comparable to uninjected controls.
  • Conventional transretinal injections resulted in significant ERG signal reduction.
  • The optimized technique minimized anatomical damage to the retina.

Conclusions:

  • Optimized minimally invasive transscleral subretinal injection is a robust technique for mice.
  • This method significantly reduces retinal damage and preserves visual function.
  • It provides a more reliable platform for evaluating gene therapies for retinal diseases.