A simple re-embedding method for the preparation of testicular cell cultures for light and electron microscopy
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Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
Published on: May 4, 2012
Journal of Microscopy
|September 1, 1985
Abstract:
A simple and reproducible method is described for the preparation of cell cultures for light and transmission electron microscopy. Mixed testicular cells are grown on collagen layered, PTFE-coated coverslips. Thin sheets of plastic-embedded cells are packed together and re-embedded permitting the examination of sections cut perpendicular to the plane of the culture and, thus, the intercellular relationships at different levels throughout the culture.

