Dual-mode fluorescence-chemiluminescence sensor for sensitive glyphosate detection based on Suc-Ce-OH BioMOF with...

Biological metal-organic frameworks (BioMOFs) demonstrate potential in sensing applications owing to their environmentally benign nature and non-toxic characteristics. In this work, a green Ce-BioMOF (Suc-Ce-OH) was synthesized via a one-step hydrothermal method using succinic acid ligands coordinated to Ce⁴⁺ metal centers. After simple alkaline treatment, the resulting Suc-Ce-OH exhibited outstanding phosphatase-like (PPA-like) activity, which could catalyze the hydrolysis of 4-methylumbellione phosphate disodium salt (4-MUP) and the dephosphorylation of 3-(2'-spiroadamantyl)-4 methoxy-4-(3'-phosphoryloxyphenyl)- 1,2-dioxetane (AMPPD) to generate blue fluorescent (FL) and strong chemiluminescence (CL) signals, respectively. Glyphosate with carboxyl group can inhibit PPA-like activity by consuming hydroxyl groups on the surface of Suc-Ce-OH, thus decrease the FL and CL intensities produced from hydrolysis of 4-MUP and AMPPD. Therefore, a FL-CL dual-mode detection method for glyphosate was established with low detection limit (LOD) of 0.081 μg mL^-1 and 0.038 μg mL^-1, respectively. This dual-mode detection method enables sensitive glyphosate detection with robust anti-interference in complex matrices and was successfully applied to the determination of glyphosate in real samples.