Related Experiment Video

Updated: Sep 11, 2025

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

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Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis

Daniel Schator1, Naren G Kumar1, Samuel Joseph U Chong1

  • 1Herbert Wertheim School of Optometry & Vision Science, University of California Berkeley, Berkeley, CA, USA.

Nature Communications
|August 11, 2025
PubMed

Insights

Pseudomonas aeruginosa bacteria cooperate between extracellular and intracellular populations to transition within host cells. This bacterial collaboration, requiring calcium influx, aids in spreading infection in vivo.

Area of Science:

  • Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Pseudomonas aeruginosa is a Gram-negative bacterium causing severe infections.
  • While traditionally extracellular, P. aeruginosa can survive and diversify within host cells.
  • Distinct intracellular subpopulations (vacuolar and cytoplasmic) have been identified.

Purpose of the Study:

  • To investigate the mechanism of P. aeruginosa transition from vacuoles to cytoplasm.
  • To explore the role of extracellular bacteria in facilitating intracellular bacterial movement.
  • To examine the contribution of bacterial cooperation to dissemination in vivo.

Main Methods:

  • Investigated calcium (Ca2+) influx.
  • Analyzed the type III secretion system (T3SS) translocon pore proteins.
  • Utilized a mouse infection model to study bacterial dissemination.

Main Results:

  • The transition of P. aeruginosa from vacuoles to cytoplasm requires extracellular bacterial collaboration.
  • Calcium influx, mediated by T3SS translocon pore proteins, is essential for this transition.
  • Cooperation between extracellular and intracellular P. aeruginosa promotes bacterial dissemination in vivo.

Conclusions:

  • Extracellular P. aeruginosa actively facilitates the intracellular relocation of other bacterial populations.
  • Bacterial cooperation and T3SS are critical for P. aeruginosa pathogenesis and persistence.
  • Understanding inter-population bacterial dynamics is key to combating P. aeruginosa infections.

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